1887

Abstract

Bacterial strain ZZ-4, a Gram-stain-negative, aerobic, non-spore-forming, non-motile, non-flagellated, rod-shaped bacterium, was isolated from tetrabromobisphenol A-contaminated soil in PR China. The taxonomic position of this strain was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain ZZ-4 was a member of the genus Emticicia and showed the highest sequence similarity to Emticicia fontis IMCC1731 (98.0 %) and Emticicia ginsengisoli Gsoil 085 (97.2 %), and lower (<97 %) sequence similarity to other known Emticicia species. Chemotaxonomic analysis revealed that strain ZZ-4 possessed menaquinone MK-7 as the major isoprenoid quinone; and iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7), iso-C17 : 0 3-OH and C16 : 1ω5c were the predominant fatty acids. Strain ZZ-4 showed low DNA–DNA relatedness with E. fontis IMCC1731 (39.8±3.1 %) and E. ginsengisoli Gsoil 085 (44.51±1.5 %). The DNA G+C content was 38.3 mol%. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data and DNA–DNA hybridization results, strain ZZ-4 is considered to represent a novel species of the genus Emticicia , for which the name Emticicia soli sp. nov. is proposed. The type strain is ZZ-4 (=KCTC 52344=CCTCC AB 2016137).

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2017-08-18
2019-10-18
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