1887

Abstract

A thermophilic bacterial strain, designated YIM 73026 was isolated from a sediment sample collected from a hot spring in Tibet, PR China. The taxonomic position of the novel isolate was investigated by a polyphasic approach. The novel isolate was Gram-stain-negative, aerobic and rod-shaped. Colonies were circular, convex, opaque and yellow. The strain grew at 50–70 °C (optimum, 60 °C), pH 6.0–8.0 (optimum, pH 7.0) and in the presence of up to 1.0 % NaCl (w/v). Comparison of the 16S rRNA gene sequences of YIM 73026 and those of other members of the genus Thermus showed sequence similarities ranging from 91.2 to 97.5 %, with YIM 73026 showing closest sequence similarity to Thermus scotoductus SE-1 (97.5 %). DNA–DNA hybridization results, however, revealed that DNA–DNA reassociation values between YIM 73026 and T. scotoductus DSM 8553 (37.6 %), Thermus amyloliquefaciens YIM 77409 (34.5 %), Thermus antranikianii DSM 12462 (30.3 %), Thermus caliditerrae YIM 77925 (28.6 %) and Thermus tengchongensis YIM 77924 (27.3 %) were well below the 70 % limit for species identification. YIM 73026 contained MK-8 as the respiratory quinone, and iso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C15 : 0 as the major cellular fatty acids (>10 %). The polar lipids consisted of one aminophospholipid, one phospholipid and two glycolipids. The genomic DNA G+C content of YIM 73026 was 65.4 mol%. On the basis of morphological, chemotaxonomic and genotypic characteristics, it is proposed that the isolate represents a novel species of the genus Thermus , for which the name Thermus caldifontis sp. nov. is proposed. The type strain is YIM 73026 (=NBRC 112415=CCTCC AB 2016305).

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2017-08-30
2019-10-19
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