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Volume 67, Issue 8

sp. nov., isolated from the rhizosphere of Mugunghwa () Free

Abstract

A Gram-stain-negative, aerobic, non-motile and coccoid to short-rod-shaped bacterial strain (THG-N2.22) was isolated from the rhizosphere of Mugunghwa (). Growth occurred at 20–40 °C (optimum 28 °C), at pH 5–9 (optimum 7) and with 0–4 % (w/v) NaCl (optimum 1 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-N2.22 were identified as YW11 (98.5 % similarity), S5 (98.5 %), ATCC 49957 (98.2 %), JC17 (97.8 %), JC288 (97.3 %) and 170/96 (97.3 %); levels of similarity with the type strains of other species were lower than 97.0 %. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, three unidentified phospholipids and three unidentified lipids. The major quinone was ubiquinone-10. The major fatty acids were C, C 2-OH, Cω7 and summed feature 3 (Cω7 and/or Cω6). The DNA G+C content of strain THG-N2.22 was 64.1 mol%. DNA–DNA hybridization values between strain THG-N2.22 and YW11, S5, ATCC 49957, JC17, JC288 and 170/96 were 43.1 % (30.2 %, reciprocal analysis), 39.0 % (24.7 %), 34.4 % (15.2 %), 18.0 % (14.5 %), 14.7 % (9.7 %) and 11.0 % (5.6 %), respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain THG-N2.22 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is THG-N2.22 (=KACC 18935=CCTCC AB 2016176).

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Keyword(s): Mugunghwa , rhizosphere soil and Roseomonas hibiscisoli
© 2017 IUMS
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2017-08-01
2024-04-18
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Roseomonas hibiscisoli sp. nov., isolated from the rhizosphere of Mugunghwa (Hibiscus syriacus)
Int J Syst Evol Microbiol 67, 2873 (2017); https://doi.org/10.1099/ijsem.0.002036
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