1887

Abstract

A Gram-stain-negative, rod-shaped, facultatively aerobic strain, motile by a monotrichous (polar or lateral) flagellum, designated BSS09, was isolated from surface sediment of the Bohai Sea, PR China. Growth was observed at 10–45 °C (optimum 32 °C), in the presence of 1.0–7.0 % (w/v) NaCl (optimum 4.0 %) and at pH 5.0–8.0 (optimum pH 6.0). Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain BSS09 belonged to the genus and showed the highest sequence similarity of 96.5 % to JCM 10544. The significant dominant fatty acid was Cω7. The polar lipids comprised one phosphatidylcholine, one phosphatidylmonomethylethanolamine, one unidentified aminolipid, one phosphatidylglycerol, one phosphatidylethanolamine and one unidentified polar lipid. The major respiratory quinone was Q-10. The DNA G+C content of strain BSS09 was 57.1 mol%. On the basis of evidence from this polyphasic study, strain BSS09 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BSS09 (=KCTC 52373=MCCC 1K03201).

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2017-08-01
2024-03-28
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References

  1. Suzuki T, Muroga Y, Takahama M, Nishimura Y. Roseigium denhamense gen. nov., sp. nov. and Roseibium hemelinense sp. nov., aerobic bacteriochlorophyll-containing bacteria isolated from the east and west coasts of Australia. Int J Syst Evol Microbiol 2000; 50:2151–2156 [View Article][PubMed]
    [Google Scholar]
  2. Biebl H, Pukall R, Lünsdorf H, Schulz S, Allgaier M et al. Description of Labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal for reclassification of Stappia aggregata as Labrenzia aggregata comb. nov., of Stappia marina as Labrenzia marina comb. nov. and of Stappia alba as Labrenzia alba comb. nov., and emended descriptions of the genera Pannonibacter, Stappia and Roseibium, and of the species Roseibium denhamense and Roseibium hamelinense. Int J Syst Evol Microbiol 2007; 50:1095–1107 [CrossRef]
    [Google Scholar]
  3. Zhong ZP, Liu Y, Liu HC, Wang F, Zhou YG et al. Roseibium aquae sp. nov., isolated from a saline lake. Int J Syst Evol Microbiol 2014; 64:2812–2818 [View Article][PubMed]
    [Google Scholar]
  4. Beveridge TJ, Lawrence JR, Murray RG. Sampling and staining for light microscopy. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology Washington, DC: American Society for Microbiology; 2007 pp. 19–33
    [Google Scholar]
  5. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  6. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology Washington, DC: American Society for Microbiology; 2007 pp. 330–393
    [Google Scholar]
  7. Yoon JH, Lee KC, Kho YH, Kang KH, Kim CJ et al. Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 2002; 52:123–130 [View Article][PubMed]
    [Google Scholar]
  8. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids,, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  9. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  10. Collins MD, Shah HN. Fatty acid, menaquinone and polar lipid composition of Rothia dentocariosa. Arch Microbiol 1984; 137:247–249 [View Article]
    [Google Scholar]
  11. Komagata K, Suzuki K-I. 4 Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1988; 19:161–207 [CrossRef]
    [Google Scholar]
  12. Xie CH, Yokota A. Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 2003; 49:345–349 [View Article][PubMed]
    [Google Scholar]
  13. Moore E, Arnscheidt A, Krüger A, Strömpl C, Mau M et al. Simplified protocols for the preparation of genomic DNA from bacterial cultures. In Akkermans ADL, van Elsas JD, de Bruijn FJ. (editors) Molecular Microbial Ecology Manual 1.6.1 Dordrecht: Kluwer; 1999 pp. 1–15
    [Google Scholar]
  14. Mesbah M, Whitman WB. Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 1989; 479:297–306 [View Article][PubMed]
    [Google Scholar]
  15. Ausubel F, Brent R, Kingston R, Moore D, Seidman J et al. Short Protocols in Molecular Biology: A Compendium of Methods From Current Protocols in Molecular Biology, 3rd ed. New York: Wiley; 1995
    [Google Scholar]
  16. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  17. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  18. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  19. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  20. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
    [Google Scholar]
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