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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 8

sp. nov., a fructose 6-phosphate phosphoketolase-positive species isolated from the oral cavity of a guinea-pig ( f. ) Free

Abstract

A slightly irregular, short rod-shaped bacterial strain, MOZIV/2, showing activity of fructose 6-phosphate phosphoketolase was isolated from the oral cavity of a home-bred guinea-pig. Based on comparative 16S rRNA gene sequence analyses, its closest relatives were DSM 21503 and DSM 17774 with 96.0 and 95.6 % pairwise similarities, respectively. Completeness of the compared sequences was 97.3 and 96.9 %, respectively. Growth was found only under anaerobic conditions. Activities of α- and β-gluco(galacto)sidases were detected in strain MOZIV/2, which is characteristic for almost all members of the family . Sequencing of other molecular markers (, and ) revealed low gene sequence similarities to DSM 21503 ranging from 72.7 to 87.5 %. Strain MOZIV/2 differed from other species within the genus by the presence of Cω9. In addition, much higher proportions of C, C, C, C, C and C fatty acids were found in cells of strain MOZIV/2. The peptidoglycan structure was of type A4α [-Lys(-Orn)–-Asp], which is consistent with its classification within the genus . The DNA G+C content (45.8 mol%) was lower than those found in other alloscardovia. Phylogenetic studies and evaluation of phenotypic characteristics including the results of biochemical, physiological and chemotaxonomic analyses confirmed the novel species status for strain MOZIV/2, for which the name sp. nov. is proposed. The type strain is MOZIV/2 (=DSM 100237=CCM 8604=LMG 28781).

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Keyword(s): Alloscardovia , Bifidobacteriaceae , guinea-pig and oral cavity
© 2017 IUMS
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2017-08-01
2024-04-25
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Alloscardovia venturai sp. nov., a fructose 6-phosphate phosphoketolase-positive species isolated from the oral cavity of a guinea-pig (Cavia aperea f. porcellus)
Int J Syst Evol Microbiol 67, 2842 (2017); https://doi.org/10.1099/ijsem.0.002031
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