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Abstract

A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium, designated strain FJAT-25547, was isolated from the purplish paddy soil collected from Linshan Township, Yanting Prefecture of Sichuan Province in PR China (31° 16′ N 105° 27′ E). Growth was achieved aerobically at temperatures between 15 and 40 °C (optimum 30 °C), with between 0 and 10.0 % NaCl (w/v) (optimum 4 %) and in the range of pH 5.0–12.0 (optimum pH 9.0). The cell-wall peptidoglycan contained meso-diaminopimelic acid, and the main isoprenoid quinone was MK-7. The major fatty acids were iso-C15 : 0 (55.4 %), anteiso-C15 : 0 (22.2 %), iso-C16 : 0 (5.1 %) and iso-C14 : 0 (6.5 %). The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FJAT-25547 was a member of the genus Bacillus and was most closely related to Bacillus horneckiae DSM 23495 (97.7 % similarity), Bacillus eiseniae A1-2 (97.5 %), Bacillus mesophilum IITR-54 (97.2 %) and Bacillus kochii WCC 4582 (97.0 %). The average nucleotide identity value between strain FJAT-25547 and the type strain of the most closely related species, B. horneckiae DSM 23495, was 77.7 %, less than the proposed cut-off value of 96.0 % for differentiating species within the genus. The in silico DNA–DNA hybridization value of strain FJAT-25547 with the most closely related species was 22.7 %, <70 %, again indicating they belong to different taxa. The DNA G+C content of strain FJAT-25547 was 39.1 mol%. This taxono-genomics study revealed that strain FJAT-25547 represents a novel species of the genus Bacillus for which the name Bacillus praedii sp. nov. (type strain FJAT-25547=CCTCC AB 2015208=DSM 101002) is proposed.

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2017-08-18
2019-10-14
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