1887

Abstract

Two aerobic, Gram-stain-negative, gliding and yellow-pigmented bacteria, designated strains 03SUJ6 and WM95 were isolated from freshwater sediment of Juam reservoir and freshwater of Woopo wetland, Republic of Korea, respectively. Cells of the two strains are motile by gliding and catalase- and oxidase-positive. The 16S rRNA gene sequence similarity between 03SUJ6 and WM95 was 97.7 %, but their DNA–DNA relatedness was 55.1 %. A maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences showed that 03SUJ6 and WM95 each form independent lineages within the genus . 03SUJ6 was related distantly to CH15-11 (97.4 % 16S rRNA gene sequence similarity), Gsoil 634 (97.3 %) and DMS 22298 (97.1 %). Closest relatives of strain WM95 were . CH15-11 (98.2 %), DSM 18422 (97.6 %), Dae 20 (97.5 %), JS5 (97.4 %) and . Gsoil 634 (97.2 %). The major fatty acids of the two isolates were summed feature 8 and C. The predominant isoprenoid quinone was ubiquinone-10. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. -Homospermidine was the major polyamine of the isolates. Phenotypic characteristics distinguished 03SUJ6 and WM95 from the related species of the genus . On the basis of the evidence presented in this study, the novel species, sp. nov. and sp. nov. are proposed for strain 03SUJ6 (=KCTC 23331=JCM 17543) and strain WM95 (=KACC 18738=JCM 31399), respectively.

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2017-08-01
2020-01-17
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