1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 8

sp. nov., a rapidly growing species related to Mycobacterium chelonae, isolated from marine teleost fish, Free

Abstract

A previously undescribed rapidly growing, non-pigmented mycobacterium was identified based on biochemical and nucleic acid analyses, as well as growth characteristics. Seven isolates were cultured from samples collected from five thread-sail filefish () and two farmed black scraper (). Bacterial growth occurred at 15–35 °C on Middlebrook 7H11 agar. The bacteria were positive for catalase activity at 68 °C and urease activity, intermediate for iron uptake, and negative for Tween 80 hydrolysis, nitrate reduction, semi-quantitative catalase activity and arylsulfatase activity at day 3. No growth was observed on Middlebrook 7H11 agar supplemented with picric acid, and very little growth was observed in the presence of 5 % NaCl. α- and α′-mycolates were identified in the cell walls, and a unique profile of the fatty acid methyl esters and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiles of the protein and cell-wall lipids were acquired. Sequence analysis revealed that the seven isolates shared identical sequences for the 16S rRNA, , , and genes. Phylogenetic analysis of the five gene sequences confirmed that the isolates were unique, but closely related to . Antibiotic susceptibility testing revealed the minimum inhibitory concentration (MIC) of clarithromycin against this novel species was <0.25 µg ml, which was lower than that for . The PCR restriction enzyme analysis pattern differed from those of and . Based on these findings, the name sp. nov. is proposed for this novel species, with the type strain being NJB0901 (=JCM 31611=KCTC 39843).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): fish pathogen , Mycobacterium chelonae - M. abscessus group , rapid-growing mycobacteria and Thread-sail filefish
© 2017 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002028
2017-08-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/8/2811.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002028&mimeType=html&fmt=ahah

References

  1. Falkinham JO. Surrounded by mycobacteria: nontuberculous mycobacteria in the human environment. J Appl Microbiol 2009; 107:356–367 [View Article][PubMed]
     [Google Scholar]
  2. Makovcova J, Slany M, Babak V, Slana I, Kralik P. The water environment as a source of potentially pathogenic mycobacteria. J Water Health 2014; 12:254–263 [View Article][PubMed]
     [Google Scholar]
  3. Hoshino Y, Suzuki K. Differential diagnostic assays for discriminating mycobacteria, especially for nontuberculous mycobacteria: what does the future hold?. Future Microbiol 2015; 10:205–216 [View Article][PubMed]
     [Google Scholar]
  4. Shivaprasad HL, Palmieri C. Pathology of mycobacteriosis in birds. Vet Clin North Am Exot Anim Pract 2012; 15:41–55 [View Article][PubMed]
     [Google Scholar]
  5. Reavill DR, Schmidt RE. Mycobacterial lesions in fish, amphibians, reptiles, rodents, lagomorphs, and ferrets with reference to animal models. Vet Clin North Am Exot Anim Pract 2012; 15:25–40 [View Article][PubMed]
     [Google Scholar]
  6. Jacobs JM, Stine CB, Baya AM, Kent ML. A review of mycobacteriosis in marine fish. J Fish Dis 2009; 32:119–130 [View Article][PubMed]
     [Google Scholar]
  7. Chemlal K, Portaels F. Molecular diagnosis of nontuberculous mycobacteria. Curr Opin Infect Dis 2003; 16:77–83 [View Article][PubMed]
     [Google Scholar]
  8. Gauthier DT, Rhodes MW. Mycobacteriosis in fishes: a review. Vet J 2009; 180:33–47 [View Article][PubMed]
     [Google Scholar]
  9. Frerichs GN. Bacterial Diseases of Fish New York: Halsted Press; 1993
     [Google Scholar]
  10. Nakanaga K, Hoshino Y, Hattori Y, Yamamoto A, Wada S et al. Mycobacterium pseudoshottsii isolated from 24 farmed fishes in western Japan. J Vet Med Sci 2012; 74:275–278 [View Article][PubMed]
     [Google Scholar]
  11. Fukano H, Wada S, Kurata O, Mizuno K, Nakanaga K et al. Nontuberculous mycobacteriosis in farmed thread-sail filefish Stephanolepis cirrhifer. Fish Pathol 2015; 50:68–74 [View Article]
     [Google Scholar]
  12. Kent PT, Kubica GP. Public Health Mycobacteriology. A Guide for the Level III Laboratory Atlanta, GA: Centers for Disease Control; 1985
     [Google Scholar]
  13. Bhatt A, Fujiwara N, Bhatt K, Gurcha SS, Kremer L et al. Deletion of kasB in Mycobacterium tuberculosis causes loss of acid-fastness and subclinical latent tuberculosis in immunocompetent mice. Proc Natl Acad Sci USA 2007; 104:5157–5162 [View Article][PubMed]
     [Google Scholar]
  14. Kim BJ, Hong SH, Kook YH, Kim BJ. Mycobacterium paragordonae sp. nov., a slowly growing, scotochromogenic species closely related to Mycobacterium gordonae. Int J Syst Evol Microbiol 2014; 64:39–45 [View Article][PubMed]
     [Google Scholar]
  15. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37:911–917 [View Article][PubMed]
     [Google Scholar]
  16. Wang C, Mahrous EA, Lee RE, Vestling MM, Takayama K. Novel polyoxyethylene-containing glycolipids are synthesized in Corynebacterium matruchotii and Mycobacterium smegmatis cultured in the presence of Tween 80. J Lipids 2011; 2011:1–12 [View Article][PubMed]
     [Google Scholar]
  17. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  18. Adékambi T, Drancourt M. Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing. Int J Syst Evol Microbiol 2004; 54:2095–2105 [View Article][PubMed]
     [Google Scholar]
  19. Clinical and Laboratory Standards Institute Susceptibility Testing of Mycobacteria, Nocardiae and Other Aerobic Actinomycetes; Approved Standard, 2nd ed. Wayne, PA: CLSI; 2011 M24-A2
    [Google Scholar]
  20. Telenti A, Marchesi F, Balz M, Bally F, Böttger EC et al. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 1993; 31:175–178[PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002028
Loading
Mycobacterium stephanolepidis sp. nov., a rapidly growing species related to Mycobacterium chelonae, isolated from marine teleost fish, Stephanolepis cirrhifer
Int J Syst Evol Microbiol 67, 2811 (2017); https://doi.org/10.1099/ijsem.0.002028
/content/journal/ijsem/10.1099/ijsem.0.002028
/content/journal/ijsem/10.1099/ijsem.0.002028
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error