1887

Abstract

A Gram-stain-negative, smooth, bright yellow-pigmented, aerobic, catalase- and oxidase-positive and rod-shaped bacterial strain was isolated from rhizosphere of Hibiscus syriacus L. (Mugunghwa flower) located in Kyung Hee University, Yongin, Gyeonggi, South Korea. Cells were dimorphic, non-motile or non-stalked, and motile by means of peritrichous flagellum. The strain, named THG-AG3.4, grew at 15–35 °C, at pH 6.5–9.0 and in the presence of 0–1.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain THG-AG3.4 was most closely related to Caulobacter segnis ATCC 21756 (98.64 % similarity), Caulobacter vibrioides CB51 (98.57 %) and Caulobacter henricii ATCC 15253 (97.41 %). The DNA G+C content of strain THG-AG3.4 was 64.0 mol%. In DNA–DNA hybridization, the DNA–DNA relatedness between strain THG-AG3.4 and its closest phylogenetic neighbour was below 55.0 %. The predominant isoprenoid quinone detected in strain THG-AG3.4 was ubiquinone-10 (Q-10). The major polar lipids were found to be an unidentified lipid, two unidentified phosphoglycolipids, five unidentified glycolipids, eight unidentified aminolipids and phosphatidylglycerol. The major fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). Thus, based on the report of the phenotypic, genotypic and phylogenetic characterization of strain THG-AG3.4, it has been concluded that the isolate represents a novel species of the genus Caulobacter , for which the name Caulobacter hibisci sp. nov. is proposed. The type strain is THG-AG3.4 (=KACC 18849=CCTCC AB 2016077).

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2017-09-04
2019-10-14
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