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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 67, Issue 9

sp. nov., isolated from rhizosphere of L. (Mugunghwa flower) Free

Abstract

A Gram-stain-negative, smooth, bright yellow-pigmented, aerobic, catalase- and oxidase-positive and rod-shaped bacterial strain was isolated from rhizosphere of L. (Mugunghwa flower) located in Kyung Hee University, Yongin, Gyeonggi, South Korea. Cells were dimorphic, non-motile or non-stalked, and motile by means of peritrichous flagellum. The strain, named THG-AG3.4, grew at 15–35 °C, at pH 6.5–9.0 and in the presence of 0–1.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain THG-AG3.4 was most closely related to ATCC 21756 (98.64 % similarity), CB51 (98.57 %) and ATCC 15253 (97.41 %). The DNA G+C content of strain THG-AG3.4 was 64.0 mol%. In DNA–DNA hybridization, the DNA–DNA relatedness between strain THG-AG3.4 and its closest phylogenetic neighbour was below 55.0 %. The predominant isoprenoid quinone detected in strain THG-AG3.4 was ubiquinone-10 (Q-10). The major polar lipids were found to be an unidentified lipid, two unidentified phosphoglycolipids, five unidentified glycolipids, eight unidentified aminolipids and phosphatidylglycerol. The major fatty acids were C, summed feature 3 (Cω and/or Cω) and summed feature 8 (C 7 and/or C 6). Thus, based on the report of the phenotypic, genotypic and phylogenetic characterization of strain THG-AG3.4, it has been concluded that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is THG-AG3.4 (=KACC 18849=CCTCC AB 2016077).

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Keyword(s): Caulobacter hibisci , Hibiscus syriacus L. , Mugunghwa flower , phenotypic analysis and rhizosphere sample
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2017-09-01
2025-04-25
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Caulobacter hibisci sp. nov., isolated from rhizosphere of Hibiscus syriacus L. (Mugunghwa flower)
Int J Syst Evol Microbiol 67, 3167 (2017); https://doi.org/10.1099/ijsem.0.002021
/content/journal/ijsem/10.1099/ijsem.0.002021
/content/journal/ijsem/10.1099/ijsem.0.002021
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