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Abstract

A Gram-stain-negative, aerobic, yellow-pigmented, non-spore-forming, non-motile, rod-shaped bacterium, designated strain DHOB07, was isolated from a soil sample collected from the lower subtropical forest of the Dinghushan Biosphere Reserve, Guangdong Province, PR China (23° 10′ N 112° 31′ E). Strain DHOB07 grew at 10–37 °C, pH 4–7 and 0–0.5 % (w/v) NaCl, with an optimum at 28 °C, pH 5–5.5 and 0% (w/v) NaCl on R2A medium. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain formed a clade with Dyella jejuensis JP1, Dyella nitratireducens DHG59, Dyella koreensis BB4, Dyella marensis CS5-B2and Dyellasoli JS12-10, with sequence similarities of 98.9, 98.0, 97.9, 97.9 and 97.8 %, respectively. Multilocus sequence analysis based on the concatenated sequences of partial housekeeping genes gyrB, lepA and recA confirmed that strain DHOB07 belongs to thegenus Dyella but is distinct from all currently known species of the genus Dyella . The G+C content of the genomic DNA was 58.2 mol%. The DNA–DNA relatedness value between strain DHOB07 and D. jejuensis JP1 was 41.8 %. Iso-C16 : 0, iso-C15 : 0 and iso-C17 : 1ω9c were the major fatty acids, and ubiquinone-8 was the only respiratory quinone detected, all of which supported the affiliation of strain DHOB07 to the genus Dyella . On the basis of the polyphasic characterization results presented above, strain DHOB07 represents a novel species of the genus Dyella , for which the name Dyella lipolytica sp. nov. is proposed. The type strain is DHOB07 (=NBRC 111473=KCTC 52132).

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2017-06-05
2019-10-13
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