1887

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Volume 67, Issue 8

gen. nov., sp. nov., a novel member of the family , phylum Free

Abstract

A Gram-stain-positive bacterium, designated TSL3, was isolated from faeces of a porcupine, , from the Budapest Zoo and Botanical Garden, Hungary. On the basis of 16S rRNA gene sequence analysis, the strain is phylogenetically related to the family . The highest 16S rRNA gene sequence similarity was found with V3M1 (96.50 %) followed by KV-653 (96.43 %). Cells of strain TSL3 were aerobic, non-motile and coccoid-shaped. The main fatty acids were anteiso-C (54.4 %), iso-C (18.2 %) and iso C (9.7 %). The major menaquinone was MK-7, and the polar lipid profile included phosphatidylglycerol, diphosphatidylglycerol, dimannosylglyceride, trimannosyldiacylglycerol, phosphatidylinositol, three unknown phospholipids and two unknown glycolipids. Strain TSL3 showed the peptidoglycan structure A4alpha -Lys - Gly - -Glu. The DNA G+C content of strain TSL3 was 58.4 mol%. Phenotypic and genotypic characterisation clearly showed that strain TSL3 could be differerentiated from the members of other genera in the family . According to these results, strain TSL3 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is TSL3 (=DSM 29785=NCAIM B. 02604).

  • Received:
  • Accepted:
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Keyword(s): 16S rDNA sequence , chemotaxonomy , Micrococcoides hystricis gen. nov., sp. nov. and phenotypic characteristics
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2017-08-01
2024-04-19
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Micrococcoides hystricis gen. nov., sp. nov., a novel member of the family Micrococcaceae, phylum Actinobacteria
Int J Syst Evol Microbiol 67, 2758 (2017); https://doi.org/10.1099/ijsem.0.002018
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