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Abstract

A novel Gram-stain-negative, aerobic marine bacterial strain, SAB-3, was isolated from brown macroalgae ( sp.) growing in the Arabian sea, Goa, India. The strain grew optimally at 30 °C, with 2.0–4.0 % (w/v) NaCl and at pH 7.0 on marine agar medium. Strain SAB-3 was unable to hydrolyse aesculin and did not grow in the presence of rifamycin but showed resistance to antibiotics such as cefadroxil and co-trimoxazole. The major fatty acids were summed feature 8 (C 7/C 6), summed feature 3 (C 7/C 6) and C, and Q-8 was the major ubiquinone. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 41.0 mol%. 16S rRNA gene sequencing and phylogenetic analysis indicated that the strain was a member of the genus with IVIA-Po-159 (97.6 % similarity), IVIA-Po-181 (97.5 %) and DSM 17202 (97.4 %) as the closest relatives. Whole genome relatedness determined through DNA–DNA hybridization revealed values of 40–50 % (below the 70 % threshold recommended for species delineation) with the above three species, thus confirming it as representing a distinct and novel species of the genus for which the name sp. nov. is proposed. The type strain is SAB-3 (=JCM 31365=KCTC 52293=MTCC 12569).

Keyword(s): 16S rRNA , FAME , MALDI-TOF , phylogeny and SAB-3T
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2017-08-01
2020-01-23
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