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Abstract

A novel Gram-staining-negative, oval-shaped (0.4–0.6×0.8–1.0 µm), non-motile strain without flagella, designated B7, was isolated from deep seawater in the South China Sea. Strain B7 was able to grow at 25–40 °C (optimum 35 °C), at pH 5.5–9.0 (optimum pH 7.0) and with 0–8 % (w/v) NaCl (optimum 3 %). Chemotaxonomic analysis showed that the predominant isoprenoid quinone was Q-10 and the dominant fatty acids were C19 : 0 cyclo 8c and summed feature 8 (C18 : 1 ω7c/C18  : 1 ω6c). The polar lipids of strain B7 were diphosphatidyglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unknown aminophospholipid, one unknown glycolipid and three unknown lipids. The DNA G+C content of the genomic DNA was 65.1 mol%. Phylogenetic analysis of 16S rRNA gene sequences showed that strain B7 belongs to the genus Mesorhizobium with similarities ranging from 96.2 to 97.5 %. Phylogenetic analyses of housekeeping genes recA, atpD and glnII indicated that strain B7 represented a distinct evolutionary lineage with the genus Mesorhizobium . OrthoANI values between strain B7 and related strains of the genus Mesorhizobium (<80 %) were lower than the threshold value of 95 % ANI relatedness for species demarcation. Therefore, strain B7 is concluded to represent a novel species of the genus Mesorhizobium , for which the name Mesorhizobium oceanicumsp. nov. is proposed. The type strain is B7 (=KCTC 42783=MCCC 1K02305).

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2017-08-30
2019-10-23
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