1887

Abstract

A mesophilic, endospore-forming, sulfate-reducing bacterium, designated strain NAW-5, was isolated from marsh soil. Cells of strain NAW-5 were Gram-stain-negative, curved rods that were motile. Strain NAW-5 grew at 18–48 °C (optimum 32–37 °C) and pH 5.8–8.4 (optimum pH 6.2–7.3). Electron donors utilized were various organic acids and H2 which support autotrophic growth. Fermentative growth occurred on carboxylic acids, but not on sugar. Sulfate, thiosulfate and elemental sulfur were used as electron acceptors. The respiratory isoprenoid quinone was MK-7. The genomic DNA G+C content of this strain was 46.6 mol%. Sequence analysis of the 16S rRNA gene showed that strain NAW-5 was affiliated to the family ‘Desulfotomaculaceae’ but the strain shared very low sequence similarity with any representatives of this family (≥89 %). Strain NAW-5 belongs to Desulfotomaculum subcluster Ig which does not include any species with validly published names. On the basis of significant differences in the phylogenetic and phenotypic properties between strain NAW-5 and related species, strain NAW-5 represents a novel species of a new genus for which the name Desulfocucumis palustris gen. nov., sp. nov. is proposed. The type strain of the type species is NAW-5 (=DSM 102911=NBRC 112242).

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2017-08-08
2019-12-07
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References

  1. Campbell LL, Postgate JR. Classification of the spore-forming sulfate-reducing bacteria. Bacteriol Rev 1965;29:359–363[PubMed]
    [Google Scholar]
  2. Kuever J, Rainey FA. Genus VII. Desulfotomaculum. In De Vos P, Garrity GM, Jones D, Krieg NR, Ludwig W et al. (editors) Bergey’s Manual of Systematic Bacteriologyvol. 3 The Firmicutes New York: Springer; pp.989–996
    [Google Scholar]
  3. Stackebrandt E. The emended family Peptococcaceae and description of the families Desulfitobacteriaceae, Desulfotomaculaceae, and Thermincolaceae. In Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F. (editors) The Prokaryotes, Firmicutes and Tenericutes Berlin, Heidelberg: Springer; 2014; pp.285–290
    [Google Scholar]
  4. Stackebrandt E, Sproer C, Rainey FA, Burghardt J, Päuker O et al. Phylogenetic analysis of the genus Desulfotomaculum: evidence for the misclassification of Desulfotomaculum guttoideum and description of Desulfotomaculum orientis as Desulfosporosinus orientis gen. nov., comb. nov. Int J Syst Bacteriol 1997;47:1134–1139 [CrossRef][PubMed]
    [Google Scholar]
  5. Kuever J, Rainey FA, Hippe H. Description of Desulfotomaculum sp. Groll as Desulfotomaculum gibsoniae sp. nov. Int J Syst Bacteriol 1999;49:1801–1808 [CrossRef][PubMed]
    [Google Scholar]
  6. Widdel F, Bak F. Gram-negative mesophilic sulfate-reducing bacteria. In Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH. (editors) The Prokaryotes, 2nd ed.vol. 4 New York: Springer-Verlag; 1992; pp.3352–3378[CrossRef]
    [Google Scholar]
  7. Muyzer G, Hottenträger S, Teske A, Wawer C. Denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA a new molecular approach to analyze the genetic diversity of mixed microbial communities. In Akkermans ADL, Van Elsas JD, De Brujin F. (editors) Molecular Microbial Ecology Manual Dordrecht: Kluwer Academic; 1996; pp.3.4.4/1–3.4.4/3
    [Google Scholar]
  8. Leifson E, Staining LE. Staining, shape and arrangement of bacterial flagella. J Bacteriol 1951;62:377[PubMed]
    [Google Scholar]
  9. Katayama-Fujimura Y, Komatsu Y, Kuraishi H, Kaneko T. Estimation of DNA base composition by high performance liquid chromatography of its nuclease PI hydrolysate. Agric Biol Chem 1984;48:3169–3172 [CrossRef]
    [Google Scholar]
  10. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959;37:911–917 [CrossRef][PubMed]
    [Google Scholar]
  11. Postgate J. A diagnostic reaction of Desulphovibrio desulphuricans. Nature 1959;183:481–482 [CrossRef][PubMed]
    [Google Scholar]
  12. Cord-Ruwisch R. A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Methods 1985;4:33–36 [CrossRef]
    [Google Scholar]
  13. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics, Chapter 6 New York: Wiley; 1991; pp.115–175
    [Google Scholar]
  14. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007;23:2947–2948 [CrossRef][PubMed]
    [Google Scholar]
  15. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  16. Wind T, Stubner S, Conrad R. Sulfate-reducing bacteria in rice field soil and on rice roots. Syst Appl Microbiol 1999;22:269–279 [CrossRef][PubMed]
    [Google Scholar]
  17. Morasch B, Schink B, Tebbe CC, Meckenstock RU. Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum. Arch Microbiol 2004;181:407–417 [CrossRef][PubMed]
    [Google Scholar]
  18. Imachi H, Sekiguchi Y, Kamagata Y, Loy A, Qiu YL et al. Non-sulfate-reducing, syntrophic bacteria affiliated with Desulfotomaculum cluster I are widely distributed in methanogenic environments. Appl Environ Microbiol 2006;72:2080–2091 [CrossRef][PubMed]
    [Google Scholar]
  19. Kaksonen AH, Spring S, Schumann P, Kroppenstedt RM, Puhakka JA. Desulfurispora thermophila gen. nov., sp. nov., a thermophilic, spore-forming sulfate-reducer isolated from a sulfidogenic fluidized-bed reactor. Int J Syst Evol Microbiol 2007;57:1089–1094 [CrossRef][PubMed]
    [Google Scholar]
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