1887

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Volume 67, Issue 8

gen. nov., sp. nov., isolated from sludge of a manganese mine Free

Abstract

A Gram-stain-positive actinobacterium, designated as strain 2-36, was isolated from sludge of a sewage outlet in a manganese mine. Phylogenetic analysis of 16S rRNA genes assigned strain 2-36 in a single lineage in the family and closely related to KLBMP 1279 (93.8 % similarity), BC444 (93.1 %) and BMG801 (92.8 %). It contained iso-C, iso-C, Cω6, iso-C and summed feature 3 (Cω6 and/or Cω7) as the major fatty acids (>5 %), with MK-9(H), MK-8(H) and MK-9(H) as the quinones. The cell wall contained -diaminopimelic acid as the diagnostic diamino acid and the DNA G+C content was 70.1 mol%. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, an unknown glycolipid, an unknown aminophospholipid and two unknown phospholipids. Compared to closely related strains, strain 2-36 showed distinguishing characteristics, such as the distinct phylogenetic lineage, positive result for phenylacetic acid assimilation and negative result for -glucosidase and having Cω6 and iso-C as the major fatty acids. On the basis of the polyphasic analyses, strain 2-36 represents a novel species of a new genus within the family Geodermatophilaceae, for which the name gen. nov., sp. nov. is proposed. The type strain of ' is 2-36 (=CCTCC AA 2016026=DSM 103787).

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  • Accepted:
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Keyword(s): Cumulibacter manganitolerans and manganese resistance
© 2017 IUMS
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2017-08-01
2024-04-16
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Cumulibacter manganitolerans gen. nov., sp. nov., isolated from sludge of a manganese mine
Int J Syst Evol Microbiol 67, 2646 (2017); https://doi.org/10.1099/ijsem.0.002004
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