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Abstract

A Gram-stain-positive, lemon yellow-pigmented, non-motile, rod-shaped bacterium, designated strain A2, was isolated from the rumen of cow. Cells were catalase-positive and weakly oxidase-positive. Growth of strain A2 was observed at 25–45 °C (optimum, 37–40 °C), at pH 5.5–9.5 (optimum, pH 7.5) and in the presence of 0–3.5 % (w/v) NaCl (optimum, 1 %). Strain A2 contained iso-C16 : 0 and anteiso-C15 : 0 as the major cellular fatty acids. Menaquinone-11 was detected as the sole respiratory quinone. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A2 formed a distinct phyletic lineage within the genus Leucobacter . Strain A2 was most closely related to ‘ Leucobacter margaritiformis ’ A23 (97.7 % 16S rRNA gene sequence similarity) and Leucobacter tardus K 70/01 (97.2 %). The major polar lipids of strain A2 were diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. Strain A2 contained a B-type cross-linked peptidoglycan based on 2,4-diaminobutyric acid as the diagnostic diamino acid with threonine, glycine, alanine and glutamic acid but lacking 4-aminobutyric acid. The G+C content of the genomic DNA was 67.0 %. From the phenotypic, chemotaxonomic and molecular features, strain A2 was considered to represent a novel species of the genus Leucobacter , for which the name Leucobacter ruminantium sp. nov. is proposed. The type strain is A2 (=KACC 17571=JCM 19316).

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2017-08-10
2019-10-20
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