sp. nov., a mesophilic sulfate reducer isolated from marine sediment Free

Abstract

Several strains of sulfate-reducing bacteria were isolated from marine sediments recovered from Hann Bay (Senegal). All were related to members of the genus . A strictly anaerobic, mesophilic and moderately halophilic strain designated BLaC1 was further characterized. Cells of strain BLaC1 stained Gram-negative and were 0.5 µm wide and 2–4 µm long, motile, rod-shaped and non-spore-forming. The four major fatty acids were anteiso-C, iso-C, iso-C and anteiso-C. Growth was observed from 15 to 45 °C (optimum 40 °C) and at pH 5.5–8 (optimum pH 7.5). The salinity range for growth was 5–65 g NaCl l (optimum 30 g l). Yeast extract was required for growth. Strain BLaC1 was able to grow on lactate and acetate in the presence of sulfate as an electron acceptor. Sulfate, thiosulfate and sulfite could serve as terminal electron acceptors, but not fumarate, nitrate or elemental sulfur. The DNA G+C content was 55.8 mol%. 16S rRNA gene sequence analysis assigned strain BLaC1 to the family ; its closest relative was DSM 19275 (93.7 % similarity). On the basis of 16S rRNA gene sequence comparisons and physiological characteristics, strain BLaC1 is proposed as representing a novel species of , with the name sp. nov. The type strain is BLaC1 (=DSM 101509=JCM 31063).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001997
2017-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/9/3162.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001997&mimeType=html&fmt=ahah

References

  1. Jørgensen BB. Mineralization of organic matter in the seabed – the role of sulfate reduction. Nature 1982; 269:643–645 [CrossRef]
    [Google Scholar]
  2. Le Gall J. A new species of Desulfovibrio. J Bacteriol 1963; 86:1120[PubMed]
    [Google Scholar]
  3. Skerman VBD, McGowan V, Sneath PHA. Approved lists of bacterial names. Int J Syst Evol Microbiol 1980; 30:225–420 [View Article]
    [Google Scholar]
  4. Campbell LL, Kasprzycki MA, Postgate JR. Desulfovibrio africans sp. n., a new dissimilatory sulfate-reducing bacterium. J Bacteriol 1966; 92:1122–1127[PubMed]
    [Google Scholar]
  5. Reichenbecher W, Schink B. Desulfovibrio inopinatus, sp. nov., a new sulfate-reducing bacterium that degrades hydroxyhydroquinone. Arch Microbiol 1997; 168:338–344 [View Article][PubMed]
    [Google Scholar]
  6. Haouari O, Fardeau ML, Casalot L, Tholozan JL, Hamdi M et al. Isolation of sulfate-reducing bacteria from Tunisian marine sediments and description of Desulfovibrio bizertensis sp. nov. Int J Syst Evol Microbiol 2006; 56:2909–2913 [View Article][PubMed]
    [Google Scholar]
  7. Vandieken V, Knoblauch C, Jørgensen BB. Desulfovibrio frigidus sp. nov. and Desulfovibrio ferrireducens sp. nov., psychrotolerant bacteria isolated from Arctic fjord sediments (Svalbard) with the ability to reduce Fe(III). Int J Syst Evol Microbiol 2006; 56:681–685 [View Article][PubMed]
    [Google Scholar]
  8. Abildgaard L, Nielsen MB, Kjeldsen KU, Ingvorsen K. Desulfovibrio alkalitolerans sp. nov., a novel alkalitolerant, sulphate-reducing bacterium isolated from district heating water. Int J Syst Evol Microbiol 2006; 56:1019–1024 [View Article][PubMed]
    [Google Scholar]
  9. Dhia Thabet OB, Fardeau ML, Suarez-Nuñez C, Hamdi M, Thomas P et al. Desulfovibrio marinus sp. nov., a moderately halophilic sulfate-reducing bacterium isolated from marine sediments in Tunisia. Int J Syst Evol Microbiol 2007; 57:2167–2170 [View Article][PubMed]
    [Google Scholar]
  10. Takii S, Hanada S, Hase Y, Tamaki H, Uyeno Y et al. Desulfovibrio marinisediminis sp. nov., a novel sulfate-reducing bacterium isolated from coastal marine sediment via enrichment with Casamino acids. Int J Syst Evol Microbiol 2008; 58:2433–2438 [View Article][PubMed]
    [Google Scholar]
  11. Suzuki D, Ueki A, Amaishi A, Ueki K. Desulfovibrio portus sp. nov., a novel sulfate-reducing bacterium in the class Deltaproteobacteria isolated from an estuarine sediment. J Gen Appl Microbiol 2009; 55:125–133 [View Article][PubMed]
    [Google Scholar]
  12. Finster KW, Kjeldsen KU. Desulfovibrio oceani subsp. oceani sp. nov., subsp. nov. and Desulfovibrio oceani subsp. galateae subsp. nov., novel sulfate-reducing bacteria isolated from the oxygen minimum zone off the coast of Peru. Antonie van Leeuwenhoek 2010; 97:221–229 [View Article][PubMed]
    [Google Scholar]
  13. Tarasov AL, Osipov GA, Borzenkov IA. Desulfovibrios from marine biofoulings at the South Vietnam coastal area and description of Desulfovibrio hontreensis sp. nov. Microbiology 2015; 84:654–664 [View Article]
    [Google Scholar]
  14. Widdel F, Pfennig N. Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. Arch Microbiol 1981; 129:395–400 [View Article]
    [Google Scholar]
  15. Hall TA. BIOEDIT: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95–98
    [Google Scholar]
  16. Maidak BL, Cole JR, Lilburn TG, Parker CT, Saxman PR et al. The RDP-II (Ribosomal Database Project). Nucleic Acids Res 2001; 29:173–174 [View Article][PubMed]
    [Google Scholar]
  17. Benson DA, Boguski MS, Lipman DJ, Ostell J, Ouellette BF et al. GenBank. Nucleic Acids Res 1999; 27:12–17 [View Article][PubMed]
    [Google Scholar]
  18. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolism vol. 3 New York: Academic Press; 1969 pp. 211–232
    [Google Scholar]
  19. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  20. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsletter 1990; 20:1–6
    [Google Scholar]
  21. Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982; 16:584–586[PubMed]
    [Google Scholar]
  22. Kuykendall LD, Roy MA, O'Neill JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 1988; 38:358–361 [View Article]
    [Google Scholar]
  23. Fardeau ML, Patel BK, Magot M, Ollivier B. Utilization of serine, leucine, isoleucine, and valine by Thermoanaerobacter brockii in the presence of thiosulfate or Methanobacterium sp. as electron acceptors. Anaerobe 1997; 3:405–410 [View Article][PubMed]
    [Google Scholar]
  24. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
  25. Claus D, Berkeley RCW. Genus Bacillus Cohn 1872. In Sneath PHA, Mair NS, Sharpe ME, Holt JG. (editors) Bergey’s Manual of Systematic Bacteriology vol. 2 Baltimore: Williams & Wilkins.; 1986 pp. 1105–1140
    [Google Scholar]
  26. Ben Ali Gam Z, Oueslati R, Abdelkafi S, Casalot L, Tholozan JL et al. Desulfovibrio tunisiensis sp. nov., a novel weakly halotolerant, sulfate-reducing bacterium isolated from exhaust water of a Tunisian oil refinery. Int J Syst Evol Microbiol 2009; 59:1059–1063 [View Article][PubMed]
    [Google Scholar]
  27. Caumette P, Cohen Y, Matheron R. Isolation and characterization of Desulfovibrio halophilus sp. nov., a halophilic sulfate-reducing bacterium isolated from Solar Lake (Sinai). Syst Appl Microbiol 1991; 14:33–38 [View Article]
    [Google Scholar]
  28. Krekeler D, Sigalevich P, Teske A, Cypionka H, Cohen Y. A sulfate-reducing bacterium from the oxic layer of a microbial mat from Solar Lake (Sinai), Desulfovibrio oxyclinae sp. nov. Arch Microbiol 1997; 167:369–375 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001997
Loading
/content/journal/ijsem/10.1099/ijsem.0.001997
Loading

Data & Media loading...

Most cited Most Cited RSS feed