1887

Abstract

A Gram-stain-negative, motile, rod-shaped bacterium designated OCN003 was cultivated from mucus taken from a diseased colony of the coral in Kāne‘ohe Bay, O‘ahu, Hawai‘i. Colonies of OCN003 were pale yellow, 1–3 mm in diameter, convex, smooth and entire. The strain was heterotrophic, strictly aerobic and strictly halophilic. Cells of OCN003 produced buds on peritrichous prosthecae. Growth occurred within the pH range of 5.5 to 10, and the temperature range of 14 to 39 °C. Major fatty acids were 16 : 1ω7, 16 : 0, 18 : 1ω7, 17 : 1ω8, 12 : 0 3-OH and 17 : 0. Phylogenetic analysis of 1399 nucleotides of the 16S rRNA gene nucleotide sequence and a multi-locus sequence analysis of three genes placed OCN003 in the genus and indicated that the nearest relatives described are , and (97–99 % sequence identity). The DNA G+C content of the strain’s genome was 40.0 mol%. Based on DNA–DNA hybridization and phenotypic differences from related type strains, we propose that OCN003 represents the type strain of a novel species in the genus , proposed as sp. nov. OCN003 (=CCOS1042=CIP 111189). An emended description of the genus is presented.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001995
2017-08-01
2024-11-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/8/2683.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001995&mimeType=html&fmt=ahah

References

  1. Gauthier G, Gauthier M, Christen R. Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int J Syst Bacteriol 1995; 45:755–761 [View Article][PubMed]
    [Google Scholar]
  2. Huang Y, Callahan S, Hadfield MG. Recruitment in the sea: bacterial genes required for inducing larval settlement in a polychaete worm. Sci Rep 2012; 2: www.nature.com/articles/srep00228 [View Article]
    [Google Scholar]
  3. Tebben J, Tapiolas DM, Motti CA, Abrego D, Negri AP et al. Induction of larval metamorphosis of the coral Acropora millepora by tetrabromopyrrole isolated from a Pseudoalteromonas bacterium. PLoS One 2011; 6:e19082 [View Article][PubMed]
    [Google Scholar]
  4. Huggett MJ, Williamson JE, de Nys R, Kjelleberg S, Steinberg PD. Larval settlement of the common australian sea urchin Heliocidaris erythrogramma in response to bacteria from the surface of coralline algae. Oecologia 2006; 149:604–619 [View Article][PubMed]
    [Google Scholar]
  5. Dahms H-U, Dobretsov S, Qian P-Y. The effect of bacterial and diatom biofilms on the settlement of the bryozoan Bugula neritina. J Exp Mar Bio Ecol 2004; 313:191–209 [View Article]
    [Google Scholar]
  6. Holmström C, Egan S, Franks A, Mccloy S, Kjelleberg S. Antifouling activities expressed by marine surface associated Pseudoalteromonas species. FEMS Microbiol Ecol 2002; 41:47–58 [View Article][PubMed]
    [Google Scholar]
  7. Pujalte MJ, Sitjà-Bobadilla A, Macián MC, Álvarez-Pellitero P, Garay E. Occurrence and virulence of Pseudoalteromonas spp. in cultured gilthead sea bream (Sparus aurata L.) and european sea bass (Dicentrarchus labrax L.). Molecular and phenotypic characterisation of P. undina strain U58. Aquaculture 2007; 271:47–53 [CrossRef]
    [Google Scholar]
  8. Nelson EJ, Ghiorse WC. Isolation and identification of Pseudoalteromonas piscicida strain Cura-d associated with diseased damselfish (Pomacentridae) eggs. J Fish Dis 1999; 22:253–260 [View Article]
    [Google Scholar]
  9. Choudhury JD, Pramanik A, Webster NS, Llewellyn LE, Gachhui R et al. The pathogen of the Great Barrier Reef Sponge Rhopaloeides odorabile is a new strain of Pseudoalteromonas agarivorans containing abundant and diverse virulence-related genes. Mar Biotechnol 2015; 17:463–478 [View Article][PubMed]
    [Google Scholar]
  10. Aebischer T, Fischer A, Walduck A, Schlötelburg C, Lindig M et al. Vaccination prevents Helicobacter pylori-induced alterations of the gastric flora in mice. FEMS Immunol Med Microbiol 2006; 46:221–229 [View Article][PubMed]
    [Google Scholar]
  11. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  12. Sawabe T, Kita-Tsukamoto K, Thompson FL. Inferring the evolutionary history of Vibrios by means of multilocus sequence analysis. J Bacteriol 2007; 189:7932–7936 [View Article][PubMed]
    [Google Scholar]
  13. Beurmann S, Videau P, Ushijima B, Smith AM, Aeby GS et al. Complete genome sequence of Pseudoalteromonas sp. strain OCN003, isolated from kāne'ohe bay, O'ahu, Hawaii. Genome Announc 2015; 3:e01396-14 [View Article][PubMed]
    [Google Scholar]
  14. Aziz RK, Bartels D, Best AA, Dejongh M, Disz T et al. The RAST server: rapid annotations using subsystems technology. BMC Genomics 2008; 9:75 [View Article][PubMed]
    [Google Scholar]
  15. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  16. Smibert R, Krieg N. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
    [Google Scholar]
  17. Mccauley EP, Haltli B, Kerr RG. Description of Pseudobacteriovorax antillogorgiicola gen. nov., sp. nov., a bacterium isolated from the gorgonian octocoral Antillogorgia elisabethae, belonging to the family Pseudobacteriovoracaceae fam. nov., within the order Bdellovibrionales. Int J Syst Evol Microbiol 2015; 65:522–530 [View Article][PubMed]
    [Google Scholar]
  18. Smith GW, Hayasaka SS. Nitrogenase activity associated with Halodule wrightii roots. Appl Environ Microbiol 1982; 43:1244–1248[PubMed]
    [Google Scholar]
  19. Aeby GS, Callahan S, Cox EF, Runyon C, Smith A et al. Emerging coral diseases in Kāne'ohe Bay, O'ahu, Hawai'i (USA): two major disease outbreaks of acute Montipora white syndrome. Dis Aquat Organ 2016; 119:189–198 [View Article][PubMed]
    [Google Scholar]
  20. Ivanova EP, Sawabe T, Lysenko AM, Gorshkova NM, Svetashev VI et al. Pseudoalteromonas ruthenica sp. nov., isolated from marine invertebrates. Int J Syst Evol Microbiol 2002; 52:235–240 [View Article][PubMed]
    [Google Scholar]
  21. Lau SC, Tsoi MM, Li X, Dobretsov S, Plakhotnikova Y et al. Pseudoalteromonas spongiae sp. nov., a novel member of the γ-Proteobacteria isolated from the sponge Mycale adhaerens in Hong Kong waters. Int J Syst Evol Microbiol 2005; 55:1593–1596 [View Article][PubMed]
    [Google Scholar]
  22. Isnansetyo A, Kamei Y. MC21-A, a bactericidal antibiotic produced by a new marine bacterium, Pseudoalteromonas phenolica sp. nov. O-BC30(T), against methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2003; 47:480–488 [View Article][PubMed]
    [Google Scholar]
  23. Gauthier MJ. Validation of the name Alteromonas luteoviolacea. Int J Syst Bacteriol 1982; 32:82–86 [View Article]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.001995
Loading
/content/journal/ijsem/10.1099/ijsem.0.001995
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error