1887

Abstract

Nine strains representing a single anamorphic novel yeast species in dispersed tropical and subtropical habitats were isolated from sugarcane leaf tissue (DMKU-SE24, DMKU-SE45, DMKU-SE129 and DMKU-SE134) and corn leaf tissue (DMKU-CE36) in Thailand, phylloplane and rhizoplane of sugarcane in Brazil (IMUFRJ 52018 and IMUFRJ 52019), bromeliad leaf tissue in Brazil (IMUFRJ 51954) and plant leaf in Japan (IPM31-24). These strains showed identical or only 1 nt substitution in the sequences of the D1/D2 region of the LSU rRNA gene and 0–5 nt substitutions in the internal transcribed spacer (ITS) region. Phylogenetic analysis based on the combined sequences of the ITS and the D1/D2 regions showed that the eight of these strains represented a single species in the genus that was distinct from related species. was the most closely related species, but with 0.9–1.2 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene, and 2.4–2.6 % nucleotide substitutions in the ITS region. They are therefore considered to represent a novel species of the genus although the formation of basidia was not observed. The name f.a., sp. nov. is proposed. The type strain is DMKU-SE45 (=CBS 14554=TBRC 6561).

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2017-08-01
2020-01-20
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References

  1. Roberts P. New heterobasidiomycetes from Great Britain. Mycotaxon 1997;63:195–216
    [Google Scholar]
  2. Sampaio JP, Bauer R, Begerow D, Oberwinkler F. Occultifur externus sp. nov., a new species of simple-pored auricularioid heterobasidiomycete from plant litter in Portugal. Mycologia 1999;91:1094–1101 [CrossRef]
    [Google Scholar]
  3. Sampaio JP, Oberwinkler F. Occultifur Oberwinkler (1990). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011; pp.1515–1518[CrossRef]
    [Google Scholar]
  4. Gomes FC, Safar SV, Marques AR, Medeiros AO, Santos AR et al. The diversity and extracellular enzymatic activities of yeasts isolated from water tanks of Vriesea minarum, an endangered bromeliad species in Brazil, and the description of Occultifur brasiliensis f.a., sp. nov. Antonie van Leeuwenhoek 2015;107:597–611 [CrossRef][PubMed]
    [Google Scholar]
  5. Khunnamwong P, Surussawadee J, Jindamorakot S, Ribeiro JR, Hagler AN et al. Occultifur tropicalis f.a., sp. nov., a novel cystobasidiomycetous yeast species isolated from tropical regions. Int J Syst Evol Microbiol 2015;65:1578–1582 [CrossRef][PubMed]
    [Google Scholar]
  6. Kurtzman CP, Robnett CJ. Occultifur kilbournensis f.a. sp. nov., a new member of the Cystobasidiales associated with maize (Zea mays) cultivation. Antonie van Leeuwenhoek 2015;107:1323–1329 [CrossRef][PubMed]
    [Google Scholar]
  7. Khunnamwong P, Surussawadee J, Jindamorakot S, Limtong S. Wickerhamiella siamensis f.a., sp. nov., an endophytic and epiphytic yeast species isolated from sugar cane leaf. Int J Syst Evol Microbiol 2014;64:3849–3855 [CrossRef][PubMed]
    [Google Scholar]
  8. Ribeiro JR, Carvalho PM, Cabral AS, Macrae A, Mendonça-Hagler LC et al. Candida middelhoveniana sp. nov., a new yeast species found on the rhizoplane of organically cultivated sugarcane. Antonie van Leeuwenhoek 2011;100:341–347 [CrossRef][PubMed]
    [Google Scholar]
  9. Takashima M, Sugita T, Van BH, Nakamura M, Endoh R et al. Taxonomic richness of yeasts in Japan within subtropical and cool temperate areas. PLoS One 2012;7:e50784 [CrossRef][PubMed]
    [Google Scholar]
  10. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998;73:331–371 [CrossRef][PubMed]
    [Google Scholar]
  11. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications New York: Academic Press; 1990; pp.315–322
    [Google Scholar]
  12. Limtong S, Yongmanitchai W, Tun MM, Kawasaki H, Seki T. Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. Int J Syst Evol Microbiol 2007;57:419–422 [CrossRef][PubMed]
    [Google Scholar]
  13. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetesapplication to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113–118 [CrossRef][PubMed]
    [Google Scholar]
  14. O’Donnell K. Fusarium and its near relatives. In Reynolds DR, Taylor JW. (editors) The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics Wallingford: CAB International; 1993; pp.226–233
    [Google Scholar]
  15. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  16. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  17. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef]
    [Google Scholar]
  18. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011; pp.87–110[CrossRef]
    [Google Scholar]
  19. Yamada Y, Kondô K. Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus, and the yeast-like genera Sporobolomyces and Rhodosporidium. J Gen Appl Microbiol 1973;19:59–77 [CrossRef]
    [Google Scholar]
  20. Kuraishi H, Katayama-Fujimura Y, Sugiyama J, Yokoyama T. Ubiquinone systems in fungi I. Distribution of ubiquinones in the major families of ascomycetes basidiomycetes and deuteromycetes and their taxonomic implications. Trans Mycol Soc Japan 1985;26:383–395
    [Google Scholar]
  21. McNeill J, Barrie FR, Buck WR, Demoulin V, Greuter W et al. International Code of Nomenclature for Algae, Fungi, and Plants (Melbourne Code)Regnum Veg 154 Koenigstein, Germany: Gantner Verlag; 2012
    [Google Scholar]
  22. Lachance MA. In defense of yeast sexual life cycles: the forma asexualis-an informal proposal. Yeast Newletter 2012;6:24–25
    [Google Scholar]
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