1887

Abstract

Three strains representing a novel yeast species were isolated from rotten wood samples collected in Xishuangbanna Tropical Rainforest in Yunnan Province, PR China. Sequence analysis of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions indicated that the novel species represented a member of the genus . The novel species was related most closely to the type strain of but they differed by 5.8 % sequence divergence (20 substitutions and 5 gaps) in the D1/D2 domain and by 12.2 % sequence divergence (27 substitutions and 15 gaps) in the ITS region. Interestingly, the novel yeast species was able to ferment glucose and sucrose in Durham tubes, a clear difference from its closest relative , the type species of the genus . The species name of sp. nov. is proposed to accommodate these strains, with NYNU 16742 (=CICC 33160=CBS 14688) designated the type strain. As the current description of the genus does not allow the inclusion of sugar fermenting species, the emendation of the diagnosis of the genus Kurtzman and Robnett is proposed.

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2017-07-01
2024-12-09
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References

  1. Middelhoven WJ. Polysaccharides and phenolic compounds as substrate for yeasts isolated from rotten wood and description of Cryptococcus fagi sp. nov. Antonie van Leeuwenhoek 2006; 90:57–67 [View Article][PubMed]
    [Google Scholar]
  2. James SA, Carvajal Barriga EJ, Bond CJ, Cross K, Núñez NC et al. Candida carvajalis sp. nov., an ascomycetous yeast species from the Ecuadorian Amazon jungle. FEMS Yeast Res 2009; 9:784–788 [View Article][PubMed]
    [Google Scholar]
  3. Wang SA, Li FL, Bai FY. Candida laoshanensis sp. nov. and Candida qingdaonensis sp. nov., anamorphic, ascomycetous yeast species isolated from decayed wood. Int J Syst Evol Microbiol 2010; 60:1697–1701 [View Article][PubMed]
    [Google Scholar]
  4. Santos RO, Cadete RM, Badotti F, Mouro A, Wallheim DO et al. Candida queiroziae sp. nov., a cellobiose-fermenting yeast species isolated from rotting wood in Atlantic rain forest. Antonie van Leeuwenhoek 2011; 99:635–642 [View Article][PubMed]
    [Google Scholar]
  5. Cadete RM, Melo MA, Lopes MR, Pereira GM, Zilli JE et al. Candida amazonensis sp. nov., an ascomycetous yeast isolated from rotting wood in the Amazonian forest. Int J Syst Evol Microbiol 2012; 62:1438–1440 [View Article][PubMed]
    [Google Scholar]
  6. Guo X, Zhu H, Bai FY. Candida cellulosicola sp. nov., a xylose-utilizing anamorphic yeast from rotten wood. Int J Syst Evol Microbiol 2012; 62:242–245 [View Article][PubMed]
    [Google Scholar]
  7. Jacques N, Sarilar V, Urien C, Lopes MR, Morais CG et al. Three novel ascomycetous yeast species of the Kazachstania clade, Kazachstania saulgeensis sp. nov., Kazachstania serrabonitensis sp. nov. and Kazachstania australis sp. nov. Reassignment of Candida humilis to Kazachstania humilis f.a. comb. nov. and Candida pseudohumilis to Kazachstania pseudohumilis f.a. comb. nov. Int J Syst Evol Microbiol 2016; 66:5192–5200 [View Article][PubMed]
    [Google Scholar]
  8. Hui FL, Chen L, Li ZH, Niu QH, Ke T. Metschnikowia henanensis sp. nov., a new anamorphic yeast species isolated from rotten wood in China. Antonie van Leeuwenhoek 2013; 103:899–904 [View Article][PubMed]
    [Google Scholar]
  9. Péter G, Dlauchy D, Tornai-Lehoczki J, Gouliamova D, Kurtzman CP. Ogataea saltuana sp. nov., a novel methanol-assimilating yeast species. Antonie van Leeuwenhoek 2011; 100:375–383 [View Article][PubMed]
    [Google Scholar]
  10. Urbina H, Blackwell M. Multilocus phylogenetic study of the Scheffersomyces yeast clade and characterization of the N-terminal region of xylose reductase gene. PLoS One 2012; 7:e39128 [View Article][PubMed]
    [Google Scholar]
  11. Morais CG, Lara CA, Borelli BM, Cadete RM, Moreira JD et al. Saturnispora bothae sp. nov., isolated from rotting wood. Int J Syst Evol Microbiol 2016; 66:3810–3813 [View Article][PubMed]
    [Google Scholar]
  12. Barbosa AC, Cadete RM, Gomes FC, Lachance MA, Rosa CA. Candida materiae sp. nov., a yeast species isolated from rotting wood in the Atlantic rain forest. Int J Syst Evol Microbiol 2009; 59:2104–2106 [View Article][PubMed]
    [Google Scholar]
  13. Cadete RM, Santos RO, Melo MA, Mouro A, Gonçalves DL et al. Spathaspora arborariae sp. nov., a d-xylose-fermenting yeast species isolated from rotting wood in Brazil. FEMS Yeast Res 2009; 9:1338–1342 [View Article][PubMed]
    [Google Scholar]
  14. Cadete RM, Melo MA, Zilli JE, Vital MJ, Mouro A et al. Spathaspora brasiliensis sp. nov., Spathaspora suhii sp. nov., Spathaspora roraimanensis sp. nov. and Spathaspora xylofermentans sp. nov., four novel d-xylose-fermenting yeast species from Brazilian Amazonian forest. Antonie van Leeuwenhoek 2013; 103:421–431 [View Article][PubMed]
    [Google Scholar]
  15. Péter G, Dlauchy D, Tornai-Lehoczki J, Suzuki M, Kurtzman CP et al. Spencermartinsiella europaea gen. nov., sp. nov., a new member of the family Trichomonascaceae. Int J Syst Evol Microbiol 2011; 61:993–1000 [View Article][PubMed]
    [Google Scholar]
  16. Morais CG, Lara CA, Oliveira ES, Péter G, Dlauchy D et al. Spencermartinsiella silvicola sp. nov., a yeast species isolated from rotting wood. Int J Syst Evol Microbiol 2016; 66:604–608 [View Article]
    [Google Scholar]
  17. Kurtzman CP, Robnett CJ. Three new anascosporic genera of the Saccharomycotina: Danielozyma gen. nov., Deakozyma gen. nov. and Middelhovenomyces gen. nov. Antonie van Leeuwenhoek 2014; 105:933–942 [View Article][PubMed]
    [Google Scholar]
  18. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts – A Taxonomic Study, 5th ed. vol. 1 Amsterdam: Elsevier; 2011 pp. 87–110 [CrossRef]
    [Google Scholar]
  19. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article][PubMed]
    [Google Scholar]
  20. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications New York: Academic Press; 1990 pp. 315–322
    [Google Scholar]
  21. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  22. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  23. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  24. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  25. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  26. Daniel HM, Vrancken G, Takrama JF, Camu N, de Vos P et al. Yeast diversity of Ghanaian cocoa bean heap fermentations. FEMS Yeast Res 2009; 9:774–783 [View Article][PubMed]
    [Google Scholar]
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