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Abstract

A bacterial strain designated STM-14 was isolated from a spring in Taiwan and characterized using a polyphasic taxonomic approach. Strain STM-14 was a Gram-stain-negative, aerobic, poly-β-hydroxybutyrate-accumulating, non-motile, rod-shaped bacterium and formed cream-coloured colonies. Strain STM-14 grew at 15–37 °C (optimum, 30 °C), at pH 5.0–9.0 (optimum, pH 6.0) and with 0–0.5 % (w/v) NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain STM-14 belonged to the genus Novosphingobium and showed the highest levels of sequence similarity to Novosphingobium naphthae D39 (98.5 %) and Novosphingobium mathurense SM117 (97.6 %). The major fatty acids (>10 %) of strain STM-14 were C17 : 1ω6c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C18 : 1ω7c, C15 : 0 2-OH and C17 : 1ω8c. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidyldimethylethanolamine, sphingoglycolipid, phosphatidylcholine and several uncharacterized lipids. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content was 66.6 mol%. The DNA–DNA hybridization value for strain STM-14 with N. naphthae JCM 31158 and N. mathurense DSM 23374 was less than 49 %. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain STM-14 should be classified as representing a novel species of the genus Novosphingobium , for which the name Novosphingobium fontis sp. nov. is proposed. The type strain is STM-14 (=BCRC 80924=LMG 29290=KCTC 42983).

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2017-07-21
2019-10-21
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