1887

Abstract

Two bacterial strains (1-10M-8-7-50 and 1M) were isolated from lagoon sediments. The strains were obligately aerobic, Gram-stain-positive, spore-forming, rod-shaped and motile. Both strains were able to grow at 25–60 ˚C (optimum 40–42 ˚C), at pH 6.0–8.8 (optimum pH 7.0–7.5) and with 0–2.0 % (w/v) (optimum 0–0.8 %) NaCl. The predominant cellular fatty acids were iso-C15 : 0 and iso-C15 : 0 2-OH, and the cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid, glycine and alanine. Phosphatidyl-N-methylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified phosphoglycolipids and three unidentified lipids were present as polar lipids. Genomic DNA G+C content was 41.9 mol%. 16S rRNA gene sequence comparisons of both strains indicated that they belong to the genus Aneurinibacillus within the family Paenibacillaceae of the class Bacilli . Both strains had a sequence similarity of 97.6 % with Aneurinibacillus migulanus B0270, 97.6 % with Aneurinibacillus aneurinilyticus ATCC 12856, 97.5 % with ‘ Aneurinibacillus humi ' U33 and <97 % with other members of the genus Aneurinibacillus . The DNA–DNA reassociation value between strain 1-10M-8-7-50 and 1M was >90 %, while strains 1-10M-8-7-50 and 1M were only 35.1±1, 29.8±1 and 24.5±1 % related to A. migulanus KACC 18173, A. aneurinilyticus KACC 18174 and ‘ A. humi ' U33, respectively. Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain 1-10M-8-7-50 as a representative of a novel species in the genus Aneurinibacillus , for which the name Aneurinibacillus sediminis sp. nov. is proposed. The type strain is 1-10M-8-7-50 (=KEMB 563-460=JCM 31819).

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2017-08-04
2019-10-18
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