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Volume 67, Issue 7

sp. nov., isolated from animal feed Free

Abstract

A Gram-stain-positive, oxidase- and catalase-positive, aerobic, rod-shaped bacterium, designated strain SK-3146, was isolated from animal feed. Phylogenetic analysis, based on 16S rRNA gene sequence comparisons, revealed that the strain formed a distinct lineage within the genus that was closely related to JCM 30953 (98.6 %), CCUG 53270 (98.0 %) and DSM 15045 (96.9 %). Cells were non-motile, endospore-forming and formed milky colonies on NA and R2A agar media. Growth of strain SK-3146 occurred at temperatures of 18–45 °C, at pH 6.0–9.5 and between 0.5–3.0 % NaCl (w/v). The major menaquinone was MK-7, with lesser amounts of MK-6 present. The cell wall peptidoglycan of strain SK-3146 contained -diaminopimelic acid. The major fatty acids were anteiso-C and iso-C. The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 53.8 mol% and the DNA–DNA hybridization relatedness values between strain SK-3146 and . JCM 30953 and . CCUG 53270 were 26.13±0.8 % and 38.7±0.6 %, respectively. The phenotypic, phylogenetic and chemotaxonomic results indicate that strain SK-3146 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SK-3146 (=KACC 18876=LMG 29568).

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Keyword(s): Firmicutes , Paenibacillus konkukensis , polyphasic taxonomy and Taxonomy
© 2017 IUMS
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2017-07-01
2024-04-19
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Paenibacillus konkukensis sp. nov., isolated from animal feed
Int J Syst Evol Microbiol 67, 2343 (2017); https://doi.org/10.1099/ijsem.0.001955
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