1887

Abstract

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and ovoid or rod-shaped bacterium, designated OITF-51, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-51 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain OITF-51 forms a cluster with the type strains of three species of the genus Flaviramulus . Strain OITF-51 exhibited 16S rRNA gene sequence similarity values of 97.0, 95.8 and 95.8 % to the type strains of Flaviramulus ichthyoenteri , Flaviramulus basaltis and Flaviramulus aquimarinus , respectively, and of less than 96.5 % to the type strains of other recognized species. Strain OITF-51 contained MK-6 as the predominant menaquinone and iso-C15 : 1 G, iso-C15 : 0, iso-C15 : 0 3-OH and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids detected in strain OITF-51 were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain OITF-51 was 33.4 mol%, and its mean DNA–DNA relatedness value with the type strain of F. ichthyoenteri was 13 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OITF-51 is separated from recognized species of the genus Flaviramulus . On the basis of the data presented, strain OITF-51 is considered to represent a novel species of the genus Flaviramulus , for which the name Flaviramulus aestuariivivens sp. nov. is proposed. The type strain is OITF-51 (=KCTC 52657=NBRC 112707).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001953
2017-07-12
2019-12-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/7/2337.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001953&mimeType=html&fmt=ahah

References

  1. Bernardet J-F. Family I. Flavobacteriaceae Reichenbach 1992. In Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ. et al. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed.vol. 4 New York: Springer; 2011; pp. 106– 111
    [Google Scholar]
  2. Einen J, Ovreås L. Flaviramulus basaltis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seafloor basalt. Int J Syst Evol Microbiol 2006; 56: 2455– 2461 [CrossRef] [PubMed]
    [Google Scholar]
  3. Zhang Y, Tang K, Shi X, Zhang XH. Flaviramulus ichthyoenteri sp. nov., an N-acylhomoserine lactone-degrading bacterium isolated from the intestine of a flounder (Paralichthys olivaceus), and emended descriptions of the genus Flaviramulus and Flaviramulus basaltis. Int J Syst Evol Microbiol 2013; 63: 4477– 4483 [CrossRef] [PubMed]
    [Google Scholar]
  4. Lee JH, Baik KS, Seong CN. Flaviramulus aquimarinus sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2015; 65: 644– 649 [CrossRef] [PubMed]
    [Google Scholar]
  5. Park S, Won SM, Kim H, Park DS, Yoon JH. Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2014; 64: 2969– 2974 [CrossRef] [PubMed]
    [Google Scholar]
  6. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50: 1861– 1868 [CrossRef] [PubMed]
    [Google Scholar]
  7. Reichenbach H. The order Cytophagales. In Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH. (editors) The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd ed. New York: Springer; 1992; pp. 3631– 3675
    [Google Scholar]
  8. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52: 1049– 1070 [CrossRef] [PubMed]
    [Google Scholar]
  9. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19: 1– 67
    [Google Scholar]
  10. Barrow G I, Feltham RKA. Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd ed. Cambridge: Cambridge University Press; 1993; [CrossRef]
    [Google Scholar]
  11. Bruns A, Rohde M, Berthe-Corti L. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 2001; 51: 1997– 2006 [CrossRef] [PubMed]
    [Google Scholar]
  12. Baumann P, Baumann L. The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG. (editors) The Prokaryotes Berlin: Springer; 1981; pp. 1302– 1331
    [Google Scholar]
  13. Staley JT. Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 1968; 95: 1921– 1942 [PubMed]
    [Google Scholar]
  14. Cohen-Bazire G, Sistrom WR, Stanier RY. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Comp Physiol 1957; 49: 25– 68 [CrossRef] [PubMed]
    [Google Scholar]
  15. Yoon JH, Kim H, Kim SB, Kim HJ, Kim WY et al. Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 1996; 46: 502– 505 [CrossRef]
    [Google Scholar]
  16. Yoon JH, Lee ST, Kim SB, Kim WY, Goodfellow M et al. Restriction fragment length polymorphism analysis of PCR-Amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains. Int J Syst Bacteriol 1997; 47: 111– 114 [CrossRef]
    [Google Scholar]
  17. Yoon JH, Kang KH, Park YH. Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 2003; 53: 449– 454 [CrossRef] [PubMed]
    [Google Scholar]
  18. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39: 224– 229 [CrossRef]
    [Google Scholar]
  19. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19: 161– 207 [CrossRef]
    [Google Scholar]
  20. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI. Inc; 1990
    [Google Scholar]
  21. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
  22. Embley TM, Wait R. Structural lipids of eubacteria. In Goodfellow M, O’Donnell AG. (editors) Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics Chichester: John Wiley & Sons; 1994; pp. 121– 161
    [Google Scholar]
  23. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25: 125– 128 [CrossRef]
    [Google Scholar]
  24. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37: 463– 464 [CrossRef]
    [Google Scholar]
  25. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44: 846– 849 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001953
Loading
/content/journal/ijsem/10.1099/ijsem.0.001953
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error