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Abstract

A bacterial strain, designated MVW-40, was isolated from Maolin Spring in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain MVW-40 were Gram-negative, strictly aerobic, motile by a single polar flagellum and bright yellow-pigmented rods with pointed ends. Growth occurred at 15–40 °C (optimum, 20–30 °C), at pH 6–9 (optimum, pH 6) and with 0–2 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MVW-40 belonged to the genus Cellvibrio and showed the highest levels of sequence similarity with respect to Cellvibrio mixtus subsp. mixtus ACM 2601 (98.1 %) and Cellvibrio fibrivorans R-4079 (97.2 %). Strain MVW-40 contained summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 1ω7c as the predominant fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, two uncharacterized aminophospholipids, two uncharacterized phospholipids and an uncharacterized lipid. The DNA G+C content of the genomic DNA was 52.8 mol%. The DNA–DNA hybridization value for strain MVW-40 with C. mixtus subsp. mixtus ACM 2601 and C. fibrivorans R-4079 was less than 45 %. On the basis of the phylogenetic inference and phenotypic data, strain MVW-40 should be classified as a novel species, for which the name Cellvibrio fontiphilus sp. nov. is proposed. The type strain is MVW-40 (=BCRC 80977=LMG 29557=KCTC 52237).

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2017-08-04
2019-12-07
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