@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001942, author = "Marcos-García, Marta and Menéndez, Esther and Ramírez-Bahena, Marta Helena and Mateos, Pedro F. and Peix, Álvaro and Velazquez, Encarna and Rivas, Raúl", title = "Mesorhizobium helmanticense sp. nov., isolated from Lotus corniculatus nodules", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "7", pages = "2301-2305", doi = "https://doi.org/10.1099/ijsem.0.001942", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001942", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "legume nodules", keywords = "Spain", keywords = "Mesorhizobium", keywords = "Lotus corniculatus", abstract = "In this study, three strains belonging to the genus Mesorhizobium , CSLC115NT, CSLC19N and CSLC37N, isolated from Lotus corniculatus nodules in Spain, were characterized. Their 16S rRNA gene sequences were closely related to those of Mesorhizobium metallidurans STM 2683T, Mesorhizobium tianshanense A-1BST, Mesorhizobium tarimense CCBAU 83306T, Mesorhizobium gobiense CCBAU 83330T and Mesorhizobium caraganae CCBAU 11299T with similarity values higher than 99.7 %. The analysis of concatenated recA and glnII genes showed that the most closely related type strains were M. metallidurans STM 2683T, M. tianshanense A-1BST and M. tarimense CCBAU 83306T with 96, 95 and 94 % similarity values in the recA gene and 95, 94 and 94 % in the glnII gene, respectively. M. metallidurans LMG 24485T, M. tianshanense USDA 3592T and M. tarimense LMG 24338T showed means of 44, 41 and 42 % DNA–DNA relatedness, respectively, with respect to strain CSLC115NT. The major fatty acids were those from summed feature 8 (C18 : 1ω7c/C18  : 1ω6c), C16 : 0 and C18 : 1ω7c 11-methyl. The results of phenotypic characterization support that the L. corniculatus nodulating strains analysed in this work belong to a novel species of the genus Mesorhizobium for which the name Mesorhizobium helmanticense sp. nov. is proposed, and the type strain is CSLC115NT (= LMG 29734T=CECT 9168T).", }