1887

Abstract

In this study, three strains belonging to the genus , CSLC115N, CSLC19N and CSLC37N, isolated from nodules in Spain, were characterized. Their 16S rRNA gene sequences were closely related to those of STM 2683, A-1BS, CCBAU 83306, CCBAU 83330 and CCBAU 11299 with similarity values higher than 99.7 %. The analysis of concatenated and genes showed that the most closely related type strains were STM 2683, A-1BS and CCBAU 83306 with 96, 95 and 94 % similarity values in the gene and 95, 94 and 94 % in the gene, respectively. LMG 24485, USDA 3592 and LMG 24338 showed means of 44, 41 and 42 % DNA–DNA relatedness, respectively, with respect to strain CSLC115N. The major fatty acids were those from summed feature 8 (Cω7/C ω6), C and Cω7 11-methyl. The results of phenotypic characterization support that the nodulating strains analysed in this work belong to a novel species of the genus for which the name sp. nov. is proposed, and the type strain is CSLC115N (= LMG 29734=CECT 9168).

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2017-07-01
2024-03-29
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