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Abstract

A Gram-staining-negative, non-motile, non-pigmented, strictly aerobic and rod-shape bacterium, designated BK296, was isolated from stream water originating from a limestone cave in Samcheok, Korea. Optimal growth of strain BK296 was observed at 30 °C, pH 7.0–8.0 and without NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BK296 belonged to the genus Perlucidibaca , forming a robust clade with a member of the genus, and was most closely related to Perlucidibaca piscinae (97.8 %). The average nucleotide identity value between strain BK296 and Perlucidibaca piscinae IMCC1704 was 79.8 %, and the genome-to-genome distance was 17.5 % on mean. The G+C content of the DNA of strain BK296 was 55.7 mol%. The major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0, C12 : 0 3-OH and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The major isoprenoid quinone was ubiquinone Q-8. On the basis of phenotypic, genotypic and phylogenetic analyses, strain BK296 (=KCTC 52162=JCM 31377) represents a novel species of the genus Perlucidibaca , for which the name Perlucidibaca aquatica sp. nov. is proposed.

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2017-07-21
2019-10-21
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