1887

Abstract

A Gram-stain-negative, aerobic, non-gliding, rod-shaped and orange-coloured bacterium, designated strain P131, was isolated from marine sediment of the coast of Weihai, China, and subjected to a polyphasic study. Strain P131 was found to grow optimally at 28–30 °C, at pH 7.0–7.5 and in the presence of 2–3 % (w/v) NaCl. In a phylogenetic analysis based on 16S rRNA gene sequences, strain P131 was found to belong to the genus Bizionia and exhibited 94.6–97.0 % 16S rRNA gene sequence similarity with recognized Bizionia species. The dominant cellular fatty acids of strain P131 were identified as iso-C15 : 0, iso-C15 : 0 G, iso-C17 : 0 3-OH and iso-C17 : 1ω9c. The predominant polar lipids were phosphatidylethanolamine, phospholipid, two aminolipids and two unidentified lipids. The predominant respiratory quinone was menaquinone MK-6 and the DNA G+C content was 36.7 mol%. On the basis of the phylogenetic and phenotypic evidence presented, strain P131 represents a novel species of the genus Bizionia , for which the name Bizionia sediminis sp. nov. is proposed. The type strain is P131 (=KCTC 42587=MCCC 1H00124).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001936
2017-07-08
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/7/2263.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001936&mimeType=html&fmt=ahah

References

  1. Nedashkovskaya OI, Kim SB, Lysenko AM, Frolova GM, Mikhailov VV et al. Bizionia paragorgiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the soft coral Paragorgia arborea. Int J Syst Evol Microbiol 2005; 55: 375– 378 [CrossRef] [PubMed]
    [Google Scholar]
  2. Bowman JP, Nichols DS. Novel members of the family Flavo bacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia. Int J Syst Evol Microbiol 2005; 55: 1471– 1486 [CrossRef] [PubMed]
    [Google Scholar]
  3. Bercovich A, Vazquez SC, Yankilevich P, Coria SH, Foti M et al. Bizionia argentinensis sp. nov., isolated from surface marine water in Antarctica. Int J Syst Evol Microbiol 2008; 58: 2363– 2367 [CrossRef] [PubMed]
    [Google Scholar]
  4. Nedashkovskaya OI, Vancanneyt M, Kim SB. Bizionia echini sp. nov., isolated from a sea urchin. Int J Syst Evol Microbiol 2010; 60: 928– 931 [CrossRef] [PubMed]
    [Google Scholar]
  5. Yoon JH, Kang CH, Jung YT, Kang SJ. Bizionia hallyeonensis sp. nov., isolated from seawater in an oyster farm. Int J Syst Evol Microbiol 2013; 63: 685– 690 [CrossRef] [PubMed]
    [Google Scholar]
  6. Song EJ, Lee MH, Seo MJ, Yim KJ, Hyun DW et al. Bizionia psychrotolerans sp. nov., a psychrophilic bacterium isolated from the intestine of a sea cucumber (Apostichopus japonicus). Antonie van Leeuwenhoek 2014; 106: 837– 844 [CrossRef] [PubMed]
    [Google Scholar]
  7. Li H, Zhang XY, Liu C, Liu A, Qin QL et al. Bizionia arctica sp. nov., isolated from Arctic fjord seawater, and emended description of the genus Bizionia. Int J Syst Evol Microbiol 2015; 65: 2925– 2930 [CrossRef] [PubMed]
    [Google Scholar]
  8. Kim HS, Hyun DW, Kim PS, Lee JY, Shin NR et al. Bizionia fulviae sp. nov., isolated from the gut of an egg cockle, Fulvia mutica. Int J Syst Evol Microbiol 2015; 65: 3066– 3072 [CrossRef] [PubMed]
    [Google Scholar]
  9. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp. 607– 654
    [Google Scholar]
  10. Bernardet JF, Nakagawa Y, Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52: 1049– 1070 [CrossRef] [PubMed]
    [Google Scholar]
  11. Dong XZ, Cai MY. (editors) Determination of biochemical characteristics. In Manual for the Systematic Identification of General Bacteria Beijing: Science Press (in Chinese); 2001; pp. 370– 398
    [Google Scholar]
  12. Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ. Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 2014; 64: 1690– 1696 [CrossRef] [PubMed]
    [Google Scholar]
  13. CLSI Performance Standards for Antimicrobial Susceptibility Testing; 22nd Informational Supplement M100-S22 Wayne, PA: Clinical and Laboratory Standards Institute; 2012
    [Google Scholar]
  14. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
  15. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982; 5: 2359– 2367 [CrossRef]
    [Google Scholar]
  16. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39: 159– 167 [CrossRef]
    [Google Scholar]
  17. Liu QQ, Li XL, Rooney AP, Du ZJ, Chen GJ. Tangfeifania diversioriginum gen. nov., sp. nov., a representative of the family Draconibacteriaceae. Int J Syst Evol Microbiol 2014; 64: 3473– 3477 [CrossRef] [PubMed]
    [Google Scholar]
  18. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62: 716– 721 [CrossRef] [PubMed]
    [Google Scholar]
  19. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25: 4876– 4882 [CrossRef] [PubMed]
    [Google Scholar]
  20. Hall TA. BioEdit: a user-friendly sequence alignment editor and analysis program for windows 95/98/NT. Nucl Acids Symp Ser 1999; 41: 95– 98
    [Google Scholar]
  21. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30: 2725– 2729 [CrossRef] [PubMed]
    [Google Scholar]
  22. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17: 368– 376 [CrossRef] [PubMed]
    [Google Scholar]
  23. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20: 406– 416 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001936
Loading
/content/journal/ijsem/10.1099/ijsem.0.001936
Loading

Data & Media loading...

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error