1887

Abstract

A Gram-stain-positive, aerobic, non-motile, rod-shaped bacterial strain, designated 2C-7, was isolated from symptomatic bark of a Populus × euramericana canker. Growth occurred between 10 and 37 °C and between pH 6 and 10, with optimal growth at 30 °C and pH 7.0–8.0. Growth was present under 0–8 % (w/v) salinity conditions (optimum 1–2 %). Growth occurred in the presence of 10 mM chromium (Cr). The major fatty acids (≥10 %) of the novel strain were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, glycolipid and two unknown lipids. The major respiratory quinone was menaquinone MK-11. The cell wall amino acids were 2,4-diaminobutyric acid, alanine, glutamic acid and glycine. Strain 2C-7 was most similar to Leucobacter celer subsp . celer NAL101 (97.2 % 16S rRNA gene sequence similarity), ‘ Leucobacter kyeonggiensis F3-P9 (97.1 %) and Leucobacter chromiireducens L-1 (97.1 %). In the phylogenetic tree, the isolate formed a single distinct branch separate from those of L. celer subsp . celer NAL101, ‘ L. kyeonggiensis’ F3-P9 and Leucobacter chironomi DSM 19883. The DNA–DNA hybridization values between the novel strain and the reference strains were lower than the accepted bacterial threshold level of 70 % for species delineation. The DNA G+C content of strain 2C-7 was 70.0 mol%. Based on the data, strain 2C-7 represents a novel species in the genus Leucobacter , for which the name Leucobacter corticis sp. nov. is proposed. The type strain is 2C-7 (=CFCC 11901=KCTC 39643).

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2017-07-12
2019-10-19
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References

  1. Takeuchi M, Weiss N, Schumann P, Yokota A. Leucobacter komagatae gen. nov., sp. nov., a new aerobic gram-positive, nonsporulating rod with 2,4-diaminobutyric acid in the cell wall. Int J Syst Bacteriol 1996; 46: 967– 971 [CrossRef] [PubMed]
    [Google Scholar]
  2. Morais PV, Paulo C, Francisco R, Branco R, Paula Chung A et al. Leucobacter luti sp. nov., and Leucobacter alluvii sp. nov., two new species of the genus Leucobacter isolated under chromium stress. Syst Appl Microbiol 2006; 29: 414– 421 [CrossRef] [PubMed]
    [Google Scholar]
  3. Shin NR, Kim MS, Jung MJ, Roh SW, Nam YD et al. Leucobacter celer sp. nov., isolated from Korean fermented seafood. Int J Syst Evol Microbiol 2011; 61: 2353– 2357 [CrossRef] [PubMed]
    [Google Scholar]
  4. Kim HJ, Lee SS. Leucobacter kyeonggiensis sp. nov., a new species isolated from dye waste water. J Microbiol 2011; 49: 1044– 1049 [CrossRef] [PubMed]
    [Google Scholar]
  5. Morais PV, Francisco R, Branco R, Chung AP, da Costa MS. Leucobacter chromiireducens sp. nov, and Leucobacter aridicollis sp. nov., two new species isolated from a chromium contaminated environment. Syst Appl Microbiol 2004; 27: 646– 652 [CrossRef] [PubMed]
    [Google Scholar]
  6. Li Y, Wang T, Piao CG, Wang LF, Tian GZ et al. Lampropedia puyangensis sp. nov., isolated from symptomatic bark of Populus × euramericana canker and emended description of Lampropedia hyalina (Ehrenberg 1832) Lee et al. 2004. Antonie van Leeuwenhoek 2015; 108: 321– 328 [CrossRef] [PubMed]
    [Google Scholar]
  7. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics New York: Wiley; 1991; pp. 115– 175
    [Google Scholar]
  8. Baker GC, Smith JJ, Cowan DA. Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 2003; 55: 541– 555 [CrossRef] [PubMed]
    [Google Scholar]
  9. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62: 716– 721 [CrossRef] [PubMed]
    [Google Scholar]
  10. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28: 2731– 2739 [CrossRef] [PubMed]
    [Google Scholar]
  11. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3: 208– 218 [CrossRef]
    [Google Scholar]
  12. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39: 159– 167 [CrossRef]
    [Google Scholar]
  13. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39: 224– 229 [CrossRef]
    [Google Scholar]
  14. Cleenwerck I, Vandemeulebroecke K, Janssens D, Swings J. Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov. Int J Syst Evol Microbiol 2002; 52: 1551– 1558 [CrossRef] [PubMed]
    [Google Scholar]
  15. Fang W, Li Y, Xue H, Tian G, Wang L et al. Corticibacter populi gen. nov., sp. nov., a new member of the family Comamonadaceae, from the bark of Populus euramericana. Int J Syst Evol Microbiol 2015; 65: 3333– 3338 [CrossRef] [PubMed]
    [Google Scholar]
  16. Moaledj K. Comparison of Gram-staining and alternate methods, KOH test and aminopeptidase activity in aquatic bacteria: their application to numerical taxonomy. J Microbiol Methods 1986; 5: 303– 310 [CrossRef]
    [Google Scholar]
  17. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Manual of Methods for General and Microbiology Washington, DC: American Society for Microbiology; 1994; pp. 607– 654
    [Google Scholar]
  18. Fang W, Li X, Tan XM, Wang LF, Piao CG et al. Leucobacter populi sp. nov. isolated from a symptomatic bark of Populus × euramericana canker. Int J Syst Evol Microbiol 2016; 66: 2254– 2258 [CrossRef] [PubMed]
    [Google Scholar]
  19. Gerhardt P, Murray R, Costilow R, Nester EW, Wood WA et al. Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology; 1981
    [Google Scholar]
  20. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. Newark: MIDI Inc; 1990
  21. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
  22. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100: 221– 230 [CrossRef] [PubMed]
    [Google Scholar]
  23. Groth I, Schumann P, Rainey FA, Martin K, Schuetze B et al. Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 1997; 47: 1129– 1133 [CrossRef] [PubMed]
    [Google Scholar]
  24. du HJ, Zhang YQ, Liu HY, Su J, Wei YZ et al. Allonocardiopsis opalescens gen. nov., sp. nov., a new member of the suborder Streptosporangineae, from the surface-sterilized fruit of a medicinal plant. Int J Syst Evol Microbiol 2013; 63: 900– 904 [CrossRef] [PubMed]
    [Google Scholar]
  25. Komagata K, Suzuki K I. Lipid and cell-wall analysis in bacterial systematics. Method Microbiol 1987; 19: 1– 67
    [Google Scholar]
  26. Halpern M, Shakéd T, Pukall R, Schumann P. Leucobacter chironomi sp. nov., a chromate-resistant bacterium isolated from a chironomid egg mass. Int J Syst Evol Microbiol 2009; 59: 665– 670 [CrossRef] [PubMed]
    [Google Scholar]
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