1887

Abstract

A Gram-stain-negative, rod-shaped, non-spore forming, motile and strictly oxidative bacterium, strain CHU3, was isolated from fresh water in the Daecheong Reservoir, South Korea. A comparison of the 16S rRNA gene sequence showed that the novel bacterium is closely related to Paucibacter toxinivorans 2C20 (=KCTC 42569) with a sequence similarity value of 97.8 %, Pelomonas saccharophila DSM 654 (=KCTC 52256) with 97.4 % similarity and Pelomonas aquatica CCUG 52575 (=KCTC 42961) with 97.3 % similarity, respectively. The major fatty acids (>10 %) of the isolate were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. Ubiquinone-8 was detected as the respiratory quinone. The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine and an unidentified aminolipid. The DNA G+C content was 62.5 mol%. DNA–DNA hybridization experiments with Paucibacter toxinivorans KCTC 42569 (=2C20), Pelomonas saccharophila KCTC 52256 (=DSM 654) and Pelomonas aquatica KCTC 42961 (=CCUG 52575) resulted in relatedness values of 20 % (reciprocal 11 %), 16 % (reciprocal 17 %) and 15 % (reciprocal 19 %), respectively. The phylogenetic analysis, DNA–DNA hybridization value, polar lipids, fatty acid composition and other physiological characteristics confirmed that strain CHU3 represents a novel species in the genus Paucibacter for which the name Paucibacter oligotrophus sp. nov. is proposed. The type strain is CHU3 (=KCTC 42519=CICC 24092). An emended description of the genus Paucibacter is also proposed on the basis of new data obtained in this study.

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2017-07-03
2019-10-16
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