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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 67, Issue 7

sp. nov., isolated from fresh water, and emended description of the genus Free

Abstract

A Gram-stain-negative, rod-shaped, non-spore forming, motile and strictly oxidative bacterium, strain CHU3, was isolated from fresh water in the Daecheong Reservoir, South Korea. A comparison of the 16S rRNA gene sequence showed that the novel bacterium is closely related to 2C20 (=KCTC 42569) with a sequence similarity value of 97.8 %, DSM 654 (=KCTC 52256) with 97.4 % similarity and CCUG 52575 (=KCTC 42961) with 97.3 % similarity, respectively. The major fatty acids (>10 %) of the isolate were summed feature 3 (Cω7 and/or Cω6) and C. Ubiquinone-8 was detected as the respiratory quinone. The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine and an unidentified aminolipid. The DNA G+C content was 62.5 mol%. DNA–DNA hybridization experiments with KCTC 42569 (=2C20), KCTC 52256 (=DSM 654) and KCTC 42961 (=CCUG 52575) resulted in relatedness values of 20 % (reciprocal 11 %), 16 % (reciprocal 17 %) and 15 % (reciprocal 19 %), respectively. The phylogenetic analysis, DNA–DNA hybridization value, polar lipids, fatty acid composition and other physiological characteristics confirmed that strain CHU3 represents a novel species in the genus for which the name sp. nov. is proposed. The type strain is CHU3 (=KCTC 42519=CICC 24092). An emended description of the genus is also proposed on the basis of new data obtained in this study.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacterial taxonomy , fresh water and polyphasic characterization
© 2017 IUMS
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2017-07-01
2025-01-26
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Paucibacter oligotrophus sp. nov., isolated from fresh water, and emended description of the genus Paucibacter
Int J Syst Evol Microbiol 67, 2231 (2017); https://doi.org/10.1099/ijsem.0.001931
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