%0 Journal Article %A Gupta, Vasundhera %A Singh, Pradip Kumar %A Korpole, Suresh %A Tanuku, Naga Radha Srinivas %A Pinnaka, Anil Kumar %T Bacillus mangrovi sp. nov., isolated from a sediment sample from a mangrove forest %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 7 %P 2219-2224 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001928 %K Firmicutes %K polyphasic taxonomy %K 16S rRNA gene %K phylogeny %K Bacillus mangrovi sp. nov. %I Microbiology Society, %X A facultatively anaerobic, endospore forming, alkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK61T, was isolated from a sediment sample collected from Coringa mangrove forest, India. Colonies were circular, 1.5 mm in diameter, shiny, smooth, yellowish and convex with entire margins after 48 h growth at 30 °C. Growth occurred at 15–42 °C, with 0–3 % (w/v) NaCl and at pH 6–9. AK61T was positive for amylase activity and negative for oxidase, catalase, aesculinase, caseinase, cellulase, DNase, gelatinase, lipase and urease activities. The fatty acids were dominated by branched types with iso- and anteiso- saturated fatty acids with a high abundance of iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0; the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid; and MK-7 was the major menaquinone. DNA–DNA hybridization between AK61T and Bacillus indicus MTCC 4374T and between AK61T and Bacillus indicus KCTC 3880 showed relatedness of 37.99 and 33.32 % respectively. The DNA G+C content of AK61T was 44 mol%. The results of a blast sequence similarity search based on 16S rRNA gene sequences indicated that Bacillus cibi and Bacillus indicus were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 97.69 and 97.55 % respectively. The results of phylogenetic analysis indicated that AK61T was clustered with Bacillus idriensis and Bacillus indicus . On the basis of its phenotypic characteristics and phylogenetic inference, AK61T represents a novel species of the genus Bacillus , for which the name Bacillus mangrovi sp. nov. is proposed. The type strain is AK61T (=JCM 31087T=MTCC 12015T=KCTC 33872T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001928