1887

Abstract

A facultatively anaerobic, endospore forming, alkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK61, was isolated from a sediment sample collected from Coringa mangrove forest, India. Colonies were circular, 1.5 mm in diameter, shiny, smooth, yellowish and convex with entire margins after 48 h growth at 30 °C. Growth occurred at 15–42 °C, with 0–3 % (w/v) NaCl and at pH 6–9. AK61 was positive for amylase activity and negative for oxidase, catalase, aesculinase, caseinase, cellulase, DNase, gelatinase, lipase and urease activities. The fatty acids were dominated by branched types with iso- and anteiso- saturated fatty acids with a high abundance of iso-C, iso-C, anteiso-C and iso-C; the cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid; and MK-7 was the major menaquinone. DNA–DNA hybridization between AK61 and MTCC 4374 and between AK61 and KCTC 3880 showed relatedness of 37.99 and 33.32 % respectively. The DNA G+C content of AK61 was 44 mol%. The results of a sequence similarity search based on 16S rRNA gene sequences indicated that and were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 97.69 and 97.55 % respectively. The results of phylogenetic analysis indicated that AK61 was clustered with and . On the basis of its phenotypic characteristics and phylogenetic inference, AK61 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AK61 (=JCM 31087=MTCC 12015=KCTC 33872).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001928
2017-07-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/7/2219.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001928&mimeType=html&fmt=ahah

References

  1. Stropko SJ, Pipes SE, Newman JD. Genome-based reclassification of Bacillus cibi as a later heterotypic synonym of Bacillus indicus and emended description of Bacillus indicus. Int J Syst Evol Microbiol 2014;64:3804–3809 [CrossRef][PubMed]
    [Google Scholar]
  2. Logan NA, Berge O, Bishop AH, Busse HJ, De Vos P et al. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 2009;59:2114–2121 [CrossRef][PubMed]
    [Google Scholar]
  3. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987;19:1–67
    [Google Scholar]
  4. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  5. Srinivas TN, Nageswara Rao SS, Vishnu Vardhan Reddy P, Pratibha MS, Sailaja B et al. Bacterial diversity and bioprospecting for cold-active lipases, amylases and proteases, from culturable bacteria of Kongsfjorden and Ny-alesund, Svalbard, Arctic. Curr Microbiol 2009;59:537–547 [CrossRef][PubMed]
    [Google Scholar]
  6. Anil Kumar P, Aravind R, Francis K, Bhumika V, Ritika C et al. Shivajiella indica gen. nov., sp. nov., a marine bacterium of the family "Cyclobacteriaceae" with nitrate reducing activity. Syst Appl Microbiol 2012;35:320–325 [CrossRef][PubMed]
    [Google Scholar]
  7. Baek SH, Cui Y, Kim SC, Cui CH, Yin C et al. Tumebacillus ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 2011;61:1715–1719 [CrossRef][PubMed]
    [Google Scholar]
  8. Sasser M. Identification of bacteria through fatty acid analysis. In Klement Z, Rudolph K, Sands DC. (editors) Methods in Phytobacteriology Budapest: Akademiai Kiado, Hungry; 1990; pp.199–204
    [Google Scholar]
  9. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in Actinomycetes and Corynebacteria. J Gen Microbiol 1977;100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  10. Groth I, Schumann P, Rainey FA, Martin K, Schuetze B et al. Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 1997;47:1129–1133 [CrossRef][PubMed]
    [Google Scholar]
  11. Komagata K, Suzuki K. Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–206[CrossRef]
    [Google Scholar]
  12. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961;3:208–218 [CrossRef]
    [Google Scholar]
  13. Sly LI, Blackall LL, Kraat PC, Tian-Shen T, Sangkhobol V. The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 1986;5:139–156 [CrossRef]
    [Google Scholar]
  14. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991; pp.115–175
    [Google Scholar]
  15. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990;215:403–410 [CrossRef][PubMed]
    [Google Scholar]
  16. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  17. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  18. Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 2004;101:11030–11035 [CrossRef][PubMed]
    [Google Scholar]
  19. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef]
    [Google Scholar]
  20. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  21. Tourova TP, Antonov AS. Identification of microorganisms by rapid DNA–DNA hybridization. Meth. Microbiol 1988;19:333–355[CrossRef]
    [Google Scholar]
  22. Rakshak K, Ravinder K, Nupur, Srinivas TN, Kumar PA. Caldimonas meghalayensis sp. nov., a novel thermophilic betaproteobacterium isolated from a hot spring of meghalaya in northeast India. Antonie van Leeuwenhoek 2013;104:1217–1225 [CrossRef][PubMed]
    [Google Scholar]
  23. Yoon JH, Lee CH, Oh TK. Bacillus cibi sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 2005;55:733–736 [CrossRef][PubMed]
    [Google Scholar]
  24. Suresh K, Prabagaran SR, Sengupta S, Shivaji S. Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India. Int J Syst Evol Microbiol 2004;54:1369–1375 [CrossRef][PubMed]
    [Google Scholar]
  25. Santos F, Peña A, Nogales B, Soria-Soria E, Del Cura MA et al. Bacterial diversity in dry modern freshwater stromatolites from Ruidera Pools Natural Park, Spain. Syst Appl Microbiol 2010;33:209–221 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001928
Loading
/content/journal/ijsem/10.1099/ijsem.0.001928
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error