1887

Abstract

A strictly aerobic, Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 3Alg 18, was isolated from the Pacific green alga Ulva fenestrata. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was affiliated to the family Flavobacteriaceae of the phylum Bacteroidetes , being most closely related to the type strains of recognized species of the genus Olleya , with 16S rRNA gene sequence similarity of 97.9–99.3 %. Strain 3Alg 18 grew in the presence of 0.5–5 % (w/v) NaCl and at 4–37 °C, and hydrolysed aesculin, casein, gelatin, starch and Tweens 20, 40 and 80. The prevalent fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH, iso-C16:0 2-OH, iso-C17 : 0 3-OH, summed feature 3, iso-C16 : 0 3-OH, anteiso-C15 : 0 and C15 : 0. The polar lipid profile contained phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The major respiratory quinone was menaquinone MK-6. The genomic DNA G+C content was 34.6 mol%. On the basis of 16S rRNA gene sequence data, and chemotaxonomic and phenotypic characteristics, strain 3Alg 18 represents a novel species of the genus Olleya , for which the name Olleya algicola sp. nov. is proposed. The type strain is 3Alg 18 (=KCTC 22024=KMM 6133).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001926
2017-07-12
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/7/2205.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001926&mimeType=html&fmt=ahah

References

  1. Nichols CM, Bowman JP, Guezennec J. Olleya marilimosa gen. nov., sp. nov., an exopolysaccharide-producing marine bacterium from the family Flavobacteriaceae, isolated from the Southern Ocean. Int J Syst Evol Microbiol 2005; 55: 1557– 1561 [CrossRef] [PubMed]
    [Google Scholar]
  2. Lee SY, Park S, Oh TK, Yoon JH. Description of Olleya aquimaris sp. nov., isolated from seawater, and emended description of the genus Olleya Mancuso Nichols et al. 2005. Int J Syst Evol Microbiol 2010; 60: 887– 891 [CrossRef] [PubMed]
    [Google Scholar]
  3. Lee MH, Jung YT, Park S, Yoon JH. Olleya namhaensis sp. nov., isolated from wood falls, and emended description of the genus Olleya Mancuso Nichols et al. 2005 emend. Lee et al. 2010. Int J Syst Evol Microbiol 2013; 63: 1610– 1615 [CrossRef] [PubMed]
    [Google Scholar]
  4. Kiselev KV, Ageenko NV, Kurilenko VV. Involvement of the cell-specific pigment genes pks and sult in bacterial defense response of sea urchins Strongylocentrotus intermedius. Dis Aquat Organ 2013; 103: 121– 132 [CrossRef] [PubMed]
    [Google Scholar]
  5. Martins P, Cleary DFR, Pires ACC, Rodrigues AM, Quintino V et al. Molecular analysis of bacterial communities and detection of potential pathogens in a recirculating aquaculture system for Scophthalmus maximus and Solea senegalensis. PLoS One 2013; 8: e80847 [CrossRef] [PubMed]
    [Google Scholar]
  6. Tropeano M, Vazquez S, Silvia Coria S, Turianski A, Cicero D et al. Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic. Pol Polar Res 2013; 34: 253– 267
    [Google Scholar]
  7. Isaac P, Sánchez LA, Bourguignon N, Cabral ME, Ferrero MA. Indigenous PAH-degrading bacteria from oil-polluted sediments in Caleta Cordova, Patagonia Argentina. Int Biodeterior Biodegr 2013; 82: 207– 214 [CrossRef]
    [Google Scholar]
  8. Lee YM, Kim SY, Jung J, Kim EH, Cho KH et al. Cultured bacterial diversity and human impact on alpine glacier cryoconite. J Microbiol 2011; 49: 355– 362 [CrossRef] [PubMed]
    [Google Scholar]
  9. Nedashkovskaya OI, Kukhlevskiy AD, Zhukova NV, Kim SB. Amylibacter ulvae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Ulva fenestrata. Arch Microbiol 2016; 198: 251– 256 [CrossRef] [PubMed]
    [Google Scholar]
  10. Nedashkovskaya OI, Kukhlevskiy AD, Zhukova NV, Kim SJ, Rhee SK et al. Winogradskyella litoriviva sp. nov., isolated from coastal seawater. Int J Syst Evol Microbiol 2015; 65: 3652– 3657 [CrossRef] [PubMed]
    [Google Scholar]
  11. Fautz E, Reichenbach H. A simple test for flexirubin-type pigments. FEMS Microbiol Lett 1980; 8: 87– 91 [CrossRef]
    [Google Scholar]
  12. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 1990; 20: 1– 6
    [Google Scholar]
  13. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37: 911– 917 [CrossRef] [PubMed]
    [Google Scholar]
  14. Collins MD, Shah HN. Fatty acid, menaquinone and polar lipid composition of Rothia dentocariosa. Arch Microbiol 1984; 137: 247– 249 [CrossRef]
    [Google Scholar]
  15. Komagata K, Suzuki K-I. Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 1987; 19: 161– 207 [CrossRef]
    [Google Scholar]
  16. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3: 208– 218 [CrossRef]
    [Google Scholar]
  17. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962; 5: 109– 118 [CrossRef] [PubMed]
    [Google Scholar]
  18. De Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970; 12: 133– 142 [CrossRef] [PubMed]
    [Google Scholar]
  19. Vancanneyt M, Naser SM, Engelbeen K, De Wachter M, Van der Meulen R et al. Reclassification of Lactobacillus brevis strains LMG 11494 and LMG 11984 as Lactobacillus parabrevis sp. nov. Int J Syst Evol Microbiol 2006; 56: 1553– 1557 [CrossRef] [PubMed]
    [Google Scholar]
  20. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62: 716– 721 [CrossRef] [PubMed]
    [Google Scholar]
  21. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22: 4673– 4680 [CrossRef] [PubMed]
    [Google Scholar]
  22. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30: 2725– 2729 [CrossRef] [PubMed]
    [Google Scholar]
  23. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406– 425 [PubMed]
    [Google Scholar]
  24. Felsenstein J. PHYLIP (phylogenetic inference package), version 3.5c. Seattle, USA: Department of Genetic, University of Washington; 1993
  25. Kluge AG, Farris JS. Quantitative phyletics and the evolution of anurans. Syst Zool 1969; 18: 1– 32 [CrossRef]
    [Google Scholar]
  26. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16: 111– 120 [CrossRef] [PubMed]
    [Google Scholar]
  27. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39: 783– 791 [CrossRef]
    [Google Scholar]
  28. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52: 1049– 1070 [CrossRef] [PubMed]
    [Google Scholar]
  29. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler P et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37: 463– 464 [CrossRef]
    [Google Scholar]
  30. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006; 33: 152– 155
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001926
Loading
/content/journal/ijsem/10.1099/ijsem.0.001926
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error