1887

Abstract

During a study of psychrophilic hydrocarbon-degrading bacteria, two yellow, Gram-staining-negative, non-motile, and rod shaped bacteria, designated R-10-9 and K-4-2 were isolated from oil-contaminated soil from Ulaanbaatar, Mongolia. Both strains were able to grow at 0–32 °C, pH 5.0–9.5, and 0–1 % (w/v) NaCl concentration. These strains were taxonomically characterized by a polyphasic approach. On the basis of the results of 16S rRNA gene sequence analysis, R-10-9 and K-4-2 belong to the genus and are closely related to 631-08 (97.80 and 98.09 % sequence similarity, respectively). The pairwise sequence similarity between R-10-9 and K-4-2 was observed to be 99.72 %. In both strains, the predominant respiratory quinone was menaquinone-6; the major polar lipid was phosphatidylethanolamine; and the major fatty acids were summed feature 3 (Cω7 and/or Cω6), iso-C, C 3-OH, antesio-C, C, iso-C 3-OH, and iso-C 3-OH. The genomic DNA G+C contents of R-10-9 and K-4-2 were 35.9 and 35.4 mol%, respectively. The DNA–DNA relatedness between R-10-9 and K-4-2 was higher than 70 % but relatedness values with closely related reference strains were less than 35 %. The morphological, physiological, chemotaxonomic, and phylogenetic analyses clearly distinguished R-10-9 from its closest phylogenetic neighbours. Thus, R-10-9 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R-10-9 (=KEMB 9005-447=KACC 18997=JCM 31673), and strain K-4-2 as an additional strain.

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2017-07-01
2020-01-28
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