1887

Abstract

A Gram-stain-negative, coccoid to oval-shaped and non-motile bacterial strain, designated MM-7, was isolated from seawater of the Yellow Sea, South Korea, and was subjected to a polyphasic taxonomic study. Strain MM-7 grew optimally at pH 7.0–8.0, at 25 °C and in the presence of 2–3 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain MM-7 joins the branch comprising the species of the genus , clustering with the type strains of and , with which it exhibited 97.9 and 96.8 % sequence similarity values, respectively. The DNA G+C content of strain MM-7 was determined to be 60.8 mol%, and its mean DNA–DNA relatedness values with JCM 21268 was 10.3±0.4 %. Strain MM-7 contained Q-10 as the predominant ubiquinone and summed feature 8 (Cω7 and/or Cω6) as the major fatty acid. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid and an unidentified lipid. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain MM-7 is distinguishable from other species of the genus . On the basis of the data presented, strain MM-7 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MM-7 (=KCTC 52469=NBRC 112431).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001922
2017-07-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/7/2189.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001922&mimeType=html&fmt=ahah

References

  1. Shiba T. Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic Pink-Pigmented Bacteria which contain bacteriochlorophyll a. Syst Appl Microbiol 1991;14:140–145 [CrossRef]
    [Google Scholar]
  2. Lafay B, Ruimy R, de Traubenberg CR, Breittmayer V, Gauthier MJ et al. Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima. Int J Syst Bacteriol 1995;45:290–296 [CrossRef][PubMed]
    [Google Scholar]
  3. Ruiz-Ponte C, Cilia V, Lambert C, Nicolas JL. Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus. Int J Syst Bacteriol 1998;48:537–542 [CrossRef][PubMed]
    [Google Scholar]
  4. Uchino Y, Hirata A, Yokota A, Sugiyama J. Reclassification of marine Agrobacterium species: proposals of Stappia stellulate gen. nov., comb. nov., Stappia aggregate sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 1998;44:201–210 [CrossRef][PubMed]
    [Google Scholar]
  5. Martens T, Heidorn T, Pukall R, Simon M, Tindall BJ et al. Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera. Int J Syst Evol Microbiol 2006;56:1293–1304 [CrossRef][PubMed]
    [Google Scholar]
  6. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000;50:1861–1868 [CrossRef][PubMed]
    [Google Scholar]
  7. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Mocrobiol 1987;19:1–67
    [Google Scholar]
  8. Barrow G I, Feltham RKA. Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd ed. Cambridge: Cambridge University Press; 1993;[CrossRef]
    [Google Scholar]
  9. Bruns A, Rohde M, Berthe-Corti L. Muricauda tuestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 2001;51:1997–2006 [CrossRef][PubMed]
    [Google Scholar]
  10. Leifson E. Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 1963;85:1183–1184[PubMed]
    [Google Scholar]
  11. Baumann P, Baumann L. The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG. (editors) The Prokaryotes Berlin: Springer; 1981; pp.1302–1331
    [Google Scholar]
  12. Cohen-Bazire G, Sistrom WR, Stanier RY. Kinetic studies of pigment synthesis by non-sulfur purple Bacteria. J Cell Comp Physiol 1957;49:25–68 [CrossRef][PubMed]
    [Google Scholar]
  13. Staley JT. Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater Bacteria. J Bacteriol 1968;95:1921–1942[PubMed]
    [Google Scholar]
  14. CLSI Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard—Eleventh Edition. CLSI Document M02-A11 Wayne, PA: Clinical and Laboratory Standards Institute; 2012
    [Google Scholar]
  15. Uchino Y, Hamada T, Yokota A. Proposal of Pseudorhodobacter ferrugineus gen nov, comb nov, for a non-photosynthetic marine bacterium, Agrobacterium ferrugineum, related to the genus Rhodobacter. J Gen Appl Microbiol 2002;48:309–319 [CrossRef][PubMed]
    [Google Scholar]
  16. Yoon J-H, Kim H, Kim S-B, Kim H-J, Kim WY et al. Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 1996;46:502–505 [CrossRef]
    [Google Scholar]
  17. Yoon JH, Lee ST, Park YH. Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 1998;48:187–194 [CrossRef][PubMed]
    [Google Scholar]
  18. Yoon JH, Kang KH, Park YH. Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 2003;53:449–454 [CrossRef][PubMed]
    [Google Scholar]
  19. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  20. Komagata K, Suzuki K. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–207[CrossRef]
    [Google Scholar]
  21. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  22. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  23. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977;27:104–117 [CrossRef]
    [Google Scholar]
  24. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984;25:125–128 [CrossRef]
    [Google Scholar]
  25. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic Acid-Deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989;39:224–229 [CrossRef]
    [Google Scholar]
  26. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
  27. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994;44:846–849 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001922
Loading
/content/journal/ijsem/10.1099/ijsem.0.001922
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error