1887

Abstract

An extremely halophilic archaeon, designated strain 5-3, was isolated from a soil sample of Meighan wetland in Iran. Strain 5-3 was strictly aerobic, catalase-positive and oxidase-negative. Cells were Gram-stain-negative, non-motile and ovoid. Colonies of strain 5-3 were cream-coloured. The isolate showed optimum growth at 4.0 M NaCl, 40 °C and pH 7.0. The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, two unknown phospholipids and three glycolipids (including one that was chromatographically identical to S-DGD). The major respiratory quinone was menaquinone MK-8. The G+C content of the genomic DNA was 61.5 mol%. The closest relative was JCM 17869 with 97.3 % similarity in the orthologous 16S rRNA gene sequence. Analysis of 16S rRNA and gene sequences indicated that strain 5-3 is a member of the genus in the family and forms a distinct cluster. On the basis of phylogenetic analysis, and phenotypic and chemotaxonomic characteristics, a novel species of the family , sp. nov., is proposed. The type strain is 5-3 (=IBRC-M 11063=LMG 29247).

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2017-07-01
2020-01-28
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