sp. nov., isolated from sea sand Free

Abstract

A Gram-stain positive, non-spore-forming, non-motile, facultatively anaerobic bacterial strain, designated CAU 1319, was isolated from sea sand and the strain’s taxonomic position was investigated using a polyphasic approach. Strain CAU 1319 grew optimally at 30 °C and at pH 7.5 in the presence of 2 % (w/v) NaCl. Phylogenetic analysis, based on the 16S rRNA gene sequence, revealed that strain CAU 1319 belongs to the genus , and is closely related to IPBSL-7 (similarity 97.69 %), Ben 106 (similarity 95.64 %) and SST-39 (similarity 95.84 %). Strain CAU 1319 had -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-9 (H) as the predominant menaquinone, and anteiso-C as the major fatty acid. The polar lipids consisted of phosphatidylglycerol, phosphatidylinositol, two unidentified aminolipids, three unidentified phospholipids and one unidentified glycolipid. Predominant polyamines were spermine and spermidine. The DNA–DNA hybridization value between strain CAU 1319 and IPBSL-7 was 24 %±0.2. The DNA G+C content of the novel strain was 69.5 mol%. On the basis of phenotypic and chemotaxonomic properties, as well as phylogenetic relatedness, strain CAU 1319should be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CAU 1319(=KCTC 39760=NBRC 111973).

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2017-06-01
2024-03-29
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