1887

Abstract

An aerobic, Gram-stain-positive, oxidase- and catalase-positive, non-motile, non-spore-forming, coccoid, creamish-white-coloured bacterium, designated strain R161, was isolated from soil in Hwaseong, South Korea. The cell-wall peptidoglycan contained glycine, glutamic acid, alanine, aspartic acid, serine and lysine, and whole-cell sugars were galactose, rhamnose, glucose and ribose. Strain R161 showed antibacterial and enzyme inhibitory activities. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain R161 formed a lineage within the family , and showed highest sequence similarity with type strains of PC IW02 (97.71 % sequence similarity) and YIM 45900 (97.58 %). The sequence similarity of strain R161 with type strains of members of the genus was less than 96.5 %. The major menaquinone was MK-8(H). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The major cellular fatty acids were iso-C, anteiso-C, iso-C H, anteiso-Cω9, summed feature 9 (iso-Cω9 and/or C 10-methyl) and iso-C. The DNA G+C content of strain R161 was 73.9 mol%. The DNA–DNA hybridization value between strain R161 and JCM 16038 was 52.1 %. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, strain R161 represents a novel species of genus , for which the name sp. nov. is proposed. The type strain of sp. nov. is R161 (=KEMB 9005-404=KACC 18906=JCM 31558).

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2017-06-01
2019-12-12
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