1887

Abstract

A bacterial strain, designated RA6, was isolated from the rhizosphere of Cistus ladanifer. Phylogenetic analyses based on 16S rRNA gene sequence placed the isolate into the genus Delftia within a cluster encompassing the type strains of Delftia lacustris , Delftia tsuruhatensis , Delftia acidovorans and Delftia litopenaei , which presented greater than 97 % sequence similarity with respect to strain RA6. DNA–DNA hybridization studies showed average relatedness ranging from of 11 to 18 % between these species of the genus Delftia and strain RA6. Catalase and oxidase were positive. Casein was hydrolysed but gelatin and starch were not. Ubiquinone 8 was the major respiratory quinone detected in strain RA6 together with low amounts of ubiquinones 7 and 9. The major fatty acids were those from summed feature 3 (C16 : 1ω7c/C16 : 1 ω6c) and C16 : 0. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain RA6 should be considered as a representative of a novel species of genus Delftia , for which the name Delftia rhizosphaerae sp. nov. is proposed. The type strain is RA6 (=LMG 29737= CECT 9171).

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2017-06-20
2019-10-21
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