Two Gram-staining-negative, aerobic bacteria (YIC 5082T and YIC4104) isolated from root nodules of Sesbania cannabina grown in a high-salt and alkaline environment were identified as a group in the genus Agrobacterium because they shared 100 and 99.7 % sequence similarities of 16S rRNA and recA+atpD genes, respectively. These two strains showed 99.2/100 % and 93.9/95.4 % 16S rRNA and recA+atpD gene sequence similarities to Agrobacterium radiobacter LMG140T and Agrobacterium. pusense NRCPB10T, respectively. The average nucleotide identities (ANI) of genome sequences were 89.95 % or lower between YIC 5082T and the species of the genus Agrobacterium examined. Moreover, these two test strains formed a unique nifH lineage deeply separated from other rhizobia. Although the nodC gene was not detected in YIC 5082T and YIC4104, they could form effective root nodules on S. cannabina plants. The main cellular fatty acids in YIC 5082T were summed feature 8 (C18 : 1ω7c/C18 : 1ω6c), C19 : 0cyclo ω8c, summed feature 2 (C12 : 0 aldehyde/unknown equivalent chain length 10.9525) and C16 : 0. The DNA G+C content of YIC 5082T was 59.3 mol%. The failure to utilize d-sorbitol as a carbon source distinguished YIC 5082T from the type strains of related species. YIC 5082T could grow in presence of 5.0 % (w/v) NaCl and at a pH of up to 10.0. Based on results regarding the genetic and phenotypic properties of YIC 5082T and YIC4104 the name Agrobacterium salinitolerans sp. nov. is proposed and YIC 5082T (=HAMBI 3646T=LMG 29287T) is designed as the type strain.
DreyfusBL, ElmerichC, DommerguesYR. Free-living Rhizobium strain able to grow on N2 as the sole nitrogen source. Appl Environ Microbiol1983; 45:711–713[PubMed]
de LajudieP, WillemsA, PotB, DewettinckD, MaestrojuanG et al. Polyphasic taxonomy of rhizobia: emendation of the genus Sinorhizobium and description of Sinorhizobium meliloti comb. nov., Sinorhizobium saheli sp. nov., and Sinorhizobium teranga sp. nov. Int J Syst Bacteriol1994; 44:715–733 [View Article]
WangET, van BerkumP, BeyeneD, SuiXH, DoradoO et al.Rhizobium huautlense sp. nov., a symbiont of Sesbania herbacea that has a close phylogenetic relationship with Rhizobium galegae. Int J Syst Bacteriol1998; 48:687–699 [View Article][PubMed]
LiX, LiY, JiangN, WuH, SongW et al. [Genetic diversity of the rhizobia and screening of high-efficient growth-promoting strains isolated from Sesbania cannabina in rudong county]. Wei Sheng Wu Xue Bao2015; 55:1105–1116[PubMed]
TerefeworkZ, KaijalainenS, LindströmK. AFLP fingerprinting as a tool to study the genetic diversity of Rhizobium galegae isolated from Galegaorientalis and Galegaofficinalis. J Biotechnol2001; 91:169–180 [View Article][PubMed]
TanZY, XuXD, WangET, GaoJL, Martinez-RomeroE et al. Phylogenetic and genetic relationships of Mesorhizobium tianshanense and related rhizobia. Int J Syst Bacteriol1997; 47:874–879 [View Article][PubMed]
HurekT, WagnerB, Reinhold-HurekB. Identification of N2-fixing plant- and fungus-associated Azoarcus species by PCR-based genomic fingerprints. Appl Environ Microbiol1997; 63:4331–4339[PubMed]
KimuraM. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol1980; 16:111–120 [View Article][PubMed]
VinuesaP, SilvaC, LoriteMJ, Izaguirre-MayoralML, BedmarEJ et al. Molecular systematics of rhizobia based on maximum likelihood and bayesian phylogenies inferred from rrs, atpD, recA and nifH sequences, and their use in the classification of Sesbania microsymbionts from Venezuelan wetlands. Syst Appl Microbiol2005; 28:702–716 [View Article][PubMed]
RichterM, Rosselló-MóraR. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA2009; 106:19126–19131 [View Article][PubMed]
KurtzS, PhillippyA, DelcherAL, SmootM, ShumwayM et al. Versatile and open software for comparing large genomes. Genome Biol2004; 5:R12 [View Article][PubMed]
YoungJM, KuykendallLD, Martínez-RomeroE, KerrA, SawadaH. A revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobiumundicola de Lajudie et al. 1998 as new combinations: Rhizobium radiobacter, R. rhizogenes, R.rubi, R. undicola and R. vitis. Int J Syst Evol Microbiol2001; 51:89–103 [View Article][PubMed]
TigheSW, de LajudieP, DipietroK, LindströmK, NickG et al. Analysis of cellular fatty acids and phenotypic relationships of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System. Int J Syst Evol Microbiol2000; 50:787–801 [View Article][PubMed]
SaritaS, SharmaPK, PrieferUB, PrellJ. Direct amplification of rhizobial nodC sequences from soil total DNA and comparison to nodC diversity of root nodule isolates. FEMS Microbiol Ecol2005; 54:1–11 [View Article][PubMed]
LaguerreG, NourSM, MacheretV, SanjuanJ, DrouinP et al. Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts. Microbiology2001; 147:981–993 [View Article][PubMed]
GaoJL, SunJG, LiY, WangET, ChenWX. Numerical taxonomy and DNA relatedness of tropical rhizobia isolated from Hainan Province, China. Int J Syst Bacteriol1994; 44:151–158 [View Article]