gen. nov., sp. nov., sp. nov. and sp. nov., new spore-forming bacteria isolated from subsurface sediments of the Baltic Sea Free

Abstract

Four novel Gram-stain-positive, endospore-forming bacteria of the order were isolated from subsurface sediments sampled during International Ocean Discovery Program Expedition 347 to the Baltic Sea. One strain (59.4M) grew as an obligate heterotroph by aerobic respiration and anaerobically by fermentation. Optimum growth was observed with 0.5 % NaCl at 25 °C and pH 7.0–7.3. Analysis of 16S rRNA gene sequences of 59.4M revealed (92.3 % identity), Geosporobacter ferrireducens (92.2 %), (91.9 %) and (91.7 %) to be the closest relatives. On the basis of the results of phenotypic and genotypic analyses, we propose that strain 59.4M represents a novel species within a novel genus, gen. nov., sp. nov., with the type strain 59.4M (=DSM 102940=JCM 31103). Three other strains, 59.4F, 59.4B and 63.6F, were affiliated with the genus and grew as strictly anaerobic sulfate reducers. These strains additionally used thiosulfate, elemental sulfur, sulfite and DMSO as electron acceptors and hydrogen as an electron donor. Strains 59.4F and 59.4B had identical 16S rRNA gene sequences, which were most similar to those of (97.8 %), (97.3 %) and (97.3 %). Strain 63.6F was closely related to (97.7 %) (96.6 %) and (96.5 %). The similarity of 16S rRNA gene sequences of strains 59.4B and 63.6F was 96.6 %. We propose the new names sp. nov., incorporating strain 59.4F (=DSM 101562=JCM 31104) and the type strain 59.4B (=DSM 101608=JCM 31105), and sp. nov., with the type strain 63.6F (=DSM 101609=JCM 31106).

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2017-06-01
2024-03-28
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