1887

Abstract

A Gram-stain-negative, aerobic, short-rod-shaped bacterium, motile by means of one flagellum (THG-T2.8), was isolated from the rhizosphere of Mugunghwa flower. Growth occurred at 10–37 °C (optimum 28 °C), at pH 6–8 (optimum 7) and with 0–5 % NaCl (optimum 1 %). The major quinone was ubiquinone-10 (Q-10). The major fatty acids were C 3-OH, C, C and C 7. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid, two unknown phospholipids, one unknown glycolipid and one unidentified lipid. The DNA G+C content of strain THG-T2.8 was 65.5 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T2.8 were identified as Tibet-S9a3 (98.6 %), B7 (98.4 %), CC-CCM15-8 (98.3 %) and JLT1284 (98.2 %). Levels of sequence similarity among strain THG-T2.8 and other species of the genus were lower than 98.0 %. DNA–DNA hybridization values between strain THG-T2.8 and Tibet-S9A3T, B7, CC-CCM15-8 and were 36.5 % (38.8 %, reciprocal analysis), 32.8 % (34.8 %), 31.6 % (33.8 %) and 15.3 % (24.8 %), respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain THG-T2.8 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is THG-T2.8 (=KACC 18932=CCTCC AB 2016181).

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2017-06-01
2024-12-14
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References

  1. Davis DH, Doudoroff M, Stanier RY, Mandel M. Proposal to reject the genus Hydrogenomonas: taxonomic implications. Int J Syst Bacteriol 1969; 19:375–390 [View Article]
    [Google Scholar]
  2. Parte AC. LPSN-list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 2014; 42:D613–D616 [View Article][PubMed]
    [Google Scholar]
  3. Kämpfer P, Lai WA, Arun AB, Young CC, Rekha PD et al. Paracoccus rhizosphaerae sp. nov., isolated from the rhizosphere of the plant Crossostephium chinense (L.) Makino (Seremban). Int J Syst Evol Microbiol 2012; 62:2750–2756 [View Article][PubMed]
    [Google Scholar]
  4. Dominguez-Moñino I, Jurado V, Hermosin B, Saiz-Jimenez C. Paracoccus cavernae sp. nov., isolated from a show cave. Int J Syst Evol Microbiol 2016; 66:2265–2270 [View Article][PubMed]
    [Google Scholar]
  5. Nakamura A. Paracoccus laeviglucosivorans sp. nov., an l-glucose-utilizing bacterium isolated from soil. Int J Syst Evol Microbiol 2015; 65:3878–3884 [View Article][PubMed]
    [Google Scholar]
  6. Park S, Yoon SY, Jung YT, Won SM, Park DS et al. Paracoccus aestuariivivens sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2016; 66:2992–2998 [View Article][PubMed]
    [Google Scholar]
  7. Roh SW, Nam YD, Chang HW, Kim KH, Kim MS et al. Paracoccus aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 2009; 59:790–794 [View Article][PubMed]
    [Google Scholar]
  8. Zhang G, Xian W, Yang J, Liu W, Jiang H et al. Paracoccus gahaiensis sp. nov. isolated from sediment of Gahai Lake, Qinghai-Tibetan Plateau, China. Arch Microbiol 2016; 198:227–232 [View Article][PubMed]
    [Google Scholar]
  9. Zheng Q, Wang Y, Chen C, Wang Y, Xia X et al. Paracoccus beibuensis sp. nov., isolated from the South China Sea. Curr Microbiol 2011; 62:710–714 [View Article][PubMed]
    [Google Scholar]
  10. Zhu S, Zhao Q, Zhang G, Jiang Z, Sheng H et al. Paracoccus tibetensis sp. nov., isolated from Qinghai-Tibet Plateau permafrost. Int J Syst Evol Microbiol 2013; 63:1902–1905 [View Article][PubMed]
    [Google Scholar]
  11. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16s ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [View Article][PubMed]
    [Google Scholar]
  12. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  13. Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  14. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95–98
    [Google Scholar]
  15. Kimura M. The Neutral Theory of Molecular Evolution UK: Cambridge University Press; 1984
    [Google Scholar]
  16. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  17. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  18. Kluge AG, Farris JS. Quantitative phyletics and the evolution of anurans. Syst Biol 1969; 18:1–32 [View Article]
    [Google Scholar]
  19. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  20. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  21. Buck JD. Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 1982; 44:992–993[PubMed]
    [Google Scholar]
  22. de Vries GE, Harms N, Maurer K, Papendrecht A, Stouthamer AH. Physiological regulation of Paracoccus denitrificans methanol dehydrogenase synthesis and activity. J Bacteriol 1988; 170:3731–3737 [View Article][PubMed]
    [Google Scholar]
  23. Yin KZ, Chen F, Li LM. Isolation of the C1-utilization ability bacteria and construction of their genomic library. Biotechnol Bull 2006; 5:23–28
    [Google Scholar]
  24. Skerman VBD. A Guide to the Identification of the Genera of Bacteria, 2nd ed. Baltimore: Williams & Wilkins; 1967
    [Google Scholar]
  25. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956; 178:703 [View Article][PubMed]
    [Google Scholar]
  26. Singh H, du J, Ngo HT, Won K, Yang JE et al. Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. isolated from rhizosphere of strawberry plant. Antonie van Leeuwenhoek 2015; 107:1437–1444 [View Article][PubMed]
    [Google Scholar]
  27. Yan ZF, Lin P, Chu X, Kook M, Li CT et al. Aeromicrobium halotolerans sp. nov., isolated from desert soil sample. Arch Microbiol 2016; 198:423–427 [View Article][PubMed]
    [Google Scholar]
  28. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
  29. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
    [Google Scholar]
  30. Stabili L, Gravili C, Tredici SM, Piraino S, Talà A et al. Epibiotic Vibrio luminous bacteria isolated from some Hydrozoa and Bryozoa species. Microb Ecol 2008; 56:625–636 [View Article][PubMed]
    [Google Scholar]
  31. Collins MD, Jones D. Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol 1980; 48:459–470 [View Article]
    [Google Scholar]
  32. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  33. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  34. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
    [Google Scholar]
  35. Hu HY, Lim BR, Goto N, Fujie K. Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples. J Microbiol Methods 2001; 47:17–24 [View Article][PubMed]
    [Google Scholar]
  36. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982; 5:2359–2367 [View Article]
    [Google Scholar]
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