1887

Abstract

gen. nov. is proposed based on isolation and phenotypic characterization of strains, predominantly from rodents. The strains showed 86 % or higher gene sequence similarity and indicated a genus-level relationship within . The strains compared at 16S rRNA gene sequence level showed 93.8 % or higher similarity, and their genus-level relationship within was confirmed by phenotypic analysis. The type species comb. nov. is reclassified from [] with type strain NCTC 8141 (=CCUG 12398). Whole genomic comparison allowed the estimation of DNA–DNA renaturation. sp. nov. was proposed for a group that included the biovar Heyl of [] with the type strain ATCC 12555 (=CCUG 998). A group was proposed as sp. nov., which included the taxon 22 of Bisgaard; the type strain is F75 (=CCUG 69665=DSM 103977). Taxon 41 of Bisgaard was proposed as sp. nov. with type strain Ac151 (=CCUG 69666=DSM 103994). sp. nov. included the type strain 1996025094 (=Ac69) (=CCUG 69667=DSM 103978). A group strains of was proposed as sp. nov. with type strain H1987082031 (=CCUG 69668=DSM 104075). Two strains including the reference strain of taxon 17 of Bisgaard that showed 16S rRNA gene similarity of 97.3 % were proposed as sp. nov. 2325/79 (=CCUG 17206=DSM 103980). sp. nov. was proposed with type strain Ppn418 (Bisgaard taxon 21) (=CCUG 69669=DSM 103979). The eight species could be separated based on phenotypic characteristics such as NAD requirement, ornithine decarboxylase and indole formation, α-glucosidase, β-galactosidase and in acid formation from (+)--arabinose, (−)--ribose, (+)--xylose, -inositol, (−)--mannitol, lactose, melibiose and trehalose. Forty-six strains including taxon 48 of Bisgaard formed a monophyletic group by and 16S rRNA gene sequence analysis, but could not be separated phenotypically from and , and it was left as an unnamed genomospecies 1 of with reference strain Ppn416. Another taxon that included 13 strains, mainly isolated from , could not be separated phenotypically from or and was designated as genomospecies 2. Strain Ppn85 with 95 % or less gene sequence similarity and with 16S rRNA gene sequence similarity of 97 % or less to the other members of was left as an unnamed singleton.

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References

  1. Jawetz E. A pneumotropic pasteurella of laboratory animals; bacteriological and serological characteristics of the organism. J Infect Dis 1950;86:172–183 [CrossRef][PubMed]
    [Google Scholar]
  2. Henriksen SD. Some Pasteurella stains from the human respiratory tract. A correction and supplement. Acta Pathologica Microbiologica Scandinavica 1962;55:355–356[CrossRef]
    [Google Scholar]
  3. Heyl JG. A study of Pasteurella strains from animal sources. Antonie van Leeuwenhoek 1963;29:79–83 [CrossRef][PubMed]
    [Google Scholar]
  4. Frederiksen W. Pasteurella taxonomy and nomenclature. In Winblad S. (editor) Yersinia, Pasteurella and Francisella, Contributions to Microbiology and Immunologyvol. 2 Basel: Karger; 1973; pp.170–176
    [Google Scholar]
  5. Mutters R, Ihm P, Pohl S, Frederiksen W, Mannheim W. Reclassification of the genus Pasteurella Trevisan 1887 on the basis of deoxyribonucleic acid homology, with proposals for the new species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis, and Pasteurella langaa. Int J Syst Bacteriol 1985;35:309–322 [CrossRef]
    [Google Scholar]
  6. Mutters R, Mannhein W, Bisgaard M. Taxonomy of the group. In Adlam C, Rutter JM. (editors) Pasteurella and Pasteurellosis London: Academic Press; 1989; pp.3–34
    [Google Scholar]
  7. Bisgaard M. Ecology and significance of Pasteurellaceae in animals. Zentralbl Bakteriol 1993;279:7–26 [CrossRef][PubMed]
    [Google Scholar]
  8. Nicklas W et al.Pasteurellaceae. In Fox JG, Davisson MT, Quimby FW, Barthold SW, Newcomer CE, Smith AL. (editors) The Mouse in Biomedical Research, 2nd ed. Philadelphia: Elsevier; 2007; pp.469–505[CrossRef]
    [Google Scholar]
  9. Nicklas W, Staut M, Benner A. Prevalence and biochemical properties of V factor-dependent Pasteurellaceae from rodents. Zentralbl Bakteriol 1993;279:114–124 [CrossRef][PubMed]
    [Google Scholar]
  10. Boot R, Thuis H, Koedam MA. Infection by V factor dependent Pasteurellaceae (Haemophilus) in rats. J Exp Animal Sci 1995;37:7–14
    [Google Scholar]
  11. Nicklas W, Baneux P, Boot R, Decelle T, Deeny AAFELASA (Federation of European Laboratory Animal Science Associations Working Group on Health Monitoring of Rodent and Rabbit Colonies) et al. Recommendations for the health monitoring of rodent and rabbit colonies in breeding and experimental units. Lab Anim 2002;36:20–42 [CrossRef][PubMed]
    [Google Scholar]
  12. Mähler M, Berard M, Feinstein R, Gallagher A, Illgen-Wilcke B et al. FELASA recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and experimental units. FELASA working group on revision of guidelines for health monitoring of rodents and rabbits. Lab Anim 2014;48:178–192[CrossRef]
    [Google Scholar]
  13. Boot R, Bisgaard M. Reclassification of 30 Pasteurellaceae strains isolated from rodents. Lab Anim 1995;29:314–319 [CrossRef][PubMed]
    [Google Scholar]
  14. Christensen H, Korczak BM, Bojesen AM, Kuhnert P, Frederiksen W et al. Classification of organisms previously reported as the SP and Stewart-Letscher groups, with descriptions of Necropsobacter gen. nov. and of Necropsobacter rosorum sp. nov. for organisms of the SP group. Int J Syst Evol Microbiol 2011;61:1829–1836 [CrossRef][PubMed]
    [Google Scholar]
  15. Christensen H, Nicklas W, Bisgaard M. Mesocricetibacter intestinalis gen. nov., sp. nov. and Cricetibacter osteomyelitidis gen. nov., sp. nov. Int J System Evol Microbiol 2014;64:3636–3643[CrossRef]
    [Google Scholar]
  16. Ryll M, Mutters R, Und Mannheim W. Untersuchungen zur genetischen Klassifikation des Pasteurella-pneumotropica-Komplexes. Berliner und Münchener Tierärztliches Wochenschrift 1991;104:243–245
    [Google Scholar]
  17. Dewhirst FE, Paster BJ, Olsen I, Fraser GJ. Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences. J Bacteriol 1992;174:2002–2013 [CrossRef][PubMed]
    [Google Scholar]
  18. Dewhirst FE, Paster BJ, Olsen I, Fraser GJ. Phylogeny of the Pasteurellaceae as determined by comparison of 16S ribosomal ribonucleic acid sequences. Zentralbl Bakteriol 1993;279:35–44 [CrossRef][PubMed]
    [Google Scholar]
  19. Christensen H, Foster G, Christensen JP, Pennycott T, Olsen JE et al. Phylogenetic analysis by 16S rDNA gene sequence comparison of avian taxa of Bisgaard and characterization and description of two new taxa of Pasteurellaceae. J Appl Microbiol 2003;95:354–363 [CrossRef][PubMed]
    [Google Scholar]
  20. Nicklas W, Bisgaard M, Aalbæk B, Kuhnert P, Christensen H. Reclassification of Actinobacillus muris as Muribacter muris gen. nov., comb. nov. Int J Syst Evol Microbiol 2015;65:3344–3351 [CrossRef][PubMed]
    [Google Scholar]
  21. Hayashimoto N, Takakura A, Itoh T. Genetic diversity on 16S rDNA sequence and phylogenic tree analysis in Pasteurella pneumotropica strains isolated from laboratory animals. Curr Microbiol 2005;51:239–243 [CrossRef][PubMed]
    [Google Scholar]
  22. Kodjo A, Villard L, Veillet F, Escande F, Borges E et al. Identification by 16S rDNA fragment amplification and determination of genetic diversity by random amplified polymorphic DNA analysis of Pasteurella pneumotropica isolated from laboratory rodents. Lab Anim Sci 1999;49:49–53[PubMed]
    [Google Scholar]
  23. Bisgaard M, Houghton SB, Mutters R, Stenzel A. Reclassification of German, British and Dutch isolates of so-called Pasteurella multocida obtained from pneumonic calf lungs. Vet Microbiol 1991;26:115–124 [CrossRef][PubMed]
    [Google Scholar]
  24. Korczak B, Christensen H, Emler S, Frey J, Kuhnert P. Phylogeny of the family Pasteurellaceae based on rpoB sequences. Int J Syst Evol Microbiol 2004;54:1393–1399 [CrossRef][PubMed]
    [Google Scholar]
  25. Korczak BM, Kuhnert P. Phylogeny of Pasteurellaceae. In Kuhnert P, Christensen H. (editors) Pasteurellaceae, Biology, Genomics and Molecular Aspects Norfolk: Caister Acad. Press; 2008; pp.27–52
    [Google Scholar]
  26. Kuhnert P, Korczak B, Falsen E, Straub R, Hoops A et al. Nicoletella semolina gen. nov., sp. nov., a new member of Pasteurellaceae isolated from horses with airway disease. J Clin Microbiol 2004;42:5542–5548 [CrossRef][PubMed]
    [Google Scholar]
  27. Angen Ø, Ahrens P, Kuhnert P, Christensen H, Mutters R. Proposal of Histophilus somni gen. nov., sp. nov. for the three species incertae sedis 'Haemophilus somnus', 'Haemophilus agni' and 'Histophilus ovis'. Int J Syst Evol Microbiol 2003;53:1449–1456 [CrossRef][PubMed]
    [Google Scholar]
  28. Christensen H, Bisgaard M, Angen O, Olsen JE. Final classification of Bisgaard taxon 9 as Actinobacillus arthritidis sp. nov. and recognition of a novel genomospecies for equine strains of Actinobacillus lignieresii. Int J Syst Evol Microbiol 2002;52:1239–1246 [CrossRef][PubMed]
    [Google Scholar]
  29. Auch AF, von Jan M, Klenk HP, Göker M. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010;2:117–134 [CrossRef][PubMed]
    [Google Scholar]
  30. Auch AF, Klenk HP, Göker M. Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand Genomic Sci 2010;2:142–148 [CrossRef][PubMed]
    [Google Scholar]
  31. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  32. Rice P, Longden I, Bleasby A. EMBOSS: the European molecular biology open software suite. Trends Genet 2000;16:276–277 [CrossRef][PubMed]
    [Google Scholar]
  33. Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007;23:2947–2948 [CrossRef][PubMed]
    [Google Scholar]
  34. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 1999;41:95–98
    [Google Scholar]
  35. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  36. Busse H-J, Bunka S, Hensel A, Lubitz W. Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 1997;47:698–708 [CrossRef]
    [Google Scholar]
  37. Kroppenstedt RM, Mannheim W. Lipoquinones in members of the family Pasteurellaceae. Int J Syst Bacteriol 1989;39:304–308 [CrossRef]
    [Google Scholar]
  38. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR, Brenner DJ, Grimont PAD et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
  39. Christensen H, Kuhnert P, Busse HJ, Frederiksen WC, Bisgaard M. Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae. Int J Syst Evol Microbiol 2007;57:166–178 [CrossRef][PubMed]
    [Google Scholar]
  40. Dole VS, Banu LA, Fister RD, Nicklas W, Henderson KS. Assessment of rpoB and 16S rRNA genes as targets for PCR-based identification of Pasteurella pneumotropica. Comp Med 2010;60:427–435[PubMed]
    [Google Scholar]
  41. Bisgaard M, Nørskov-Lauritsen N, de Wit SJ, Hess C, Christensen H. Multilocus sequence phylogenetic analysis of Avibacterium. Microbiology 2012;158:993–1004 [CrossRef][PubMed]
    [Google Scholar]
  42. Christensen H, Kuhnert P, Nørskov-Lauritsen N, Planet PJ, Bisgaard M et al. Family Pasteurellaceae. In Stackebrandt E, Rosenberg E, Delong E, Lory S, Thompson F et al. (editors) The Prokaryotes, 4th ed.vol. 9 New York: Springer; 2014; pp.535–564
    [Google Scholar]
  43. Octavia S, Lan R. The family Enterobacteriaceae. In Stackebrandt E, Rosenberg E, Delong E, Lory S, Thompson F et al. (editors) The Prokaryotes, 4th ed.vol. 9 New York: Springer; 2014; pp.223–286
    [Google Scholar]
  44. Stackebrandt E, Frederiksen W, Garrity GM, Grimont PA, Kämpfer P et al. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 2002;52:1043–1047 [CrossRef][PubMed]
    [Google Scholar]
  45. Hedegaard J, Okkels H, Bruun B, Kilian M, Mortensen KK et al. Phylogeny of the genus Haemophilus as determined by comparison of partial infB sequences. Microbiology 2001;147:2599–2609 [CrossRef][PubMed]
    [Google Scholar]
  46. Kudirkiene E, Welker M, Knudsen NR, Bojesen AM. Rapid and accurate identification of Streptococcus equi subspecies by MALDI-TOF MS. Syst Appl Microbiol 2015;38:315–322 [CrossRef][PubMed]
    [Google Scholar]
  47. Bisgaard M. Actinobacillus muris sp. nov. isolated from mice. Acta Pathol Microbiol Immunol Scand B 1986;94:1–8 [CrossRef][PubMed]
    [Google Scholar]
  48. Kilian M. Genus III. Haemophilus Winslow, Broadhurst, Buchanan, Krumwiede, Rogers and Smith 1917, 561AL. In Brenner DJ, Krieg NR, Staley JT, Garrity G. (editors) Bergey's Manual of Systematic Bacteriology The Proteobacteria. Part B the Gammaproteobacteria, 2nd ed. New York: Springer; 2005; pp.883–904
    [Google Scholar]
  49. Nørskov-Lauritsen N, Bruun B, Kilian M. Multilocus sequence phylogenetic study of the genus Haemophilus with description of Haemophilus pittmaniae sp. nov. Int J Syst Evol Microbiol 2005;55:449–456 [CrossRef][PubMed]
    [Google Scholar]
  50. Winslow CE, Broadhurst J, Buchanan RE, Krumwiede C, Rogers LA et al. The families and genera of the bacteria: preliminary report of the committee of the society of American bacteriologists on characterization and classification of bacterial types. J Bacteriol 1917;2:505–566[PubMed]
    [Google Scholar]
  51. Zinnemann K, Biberstein EL. Genus Haemophilus Winslow, Broadhurst, Buchanan, Krumwiede, Rogers and Smith 1917, 561. In Buchanan RE, Gibbons NE. (editors) Bergeys’s Manual of Determinative Bacteriology Legends for Figures, 8th ed. Baltimore: Williams and Wilkins; 1974; pp.364–370
    [Google Scholar]
  52. Brumpt E. Précis De Parasitologie Paris: Masson et Cie; 1910
    [Google Scholar]
  53. Christensen H, Bisgaard M. Revised definition of Actinobacillus sensu stricto isolated from animals: a review with special emphasis on diagnosis. Vet Microbiol 2004;99:13–30 [CrossRef][PubMed]
    [Google Scholar]
  54. Osawa R, Rainey F, Fujisawa T, Lang E, Busse HJ et al. Lonepinella koalarum gen. nov., sp. nov., a new tannin-protein complex degrading bacterium. Syst Appl Microbiol 1995;18:368–373 [CrossRef]
    [Google Scholar]
  55. Angen O, Mutters R, Caugant DA, Olsen JE, Bisgaard M. Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov. Int J Syst Bacteriol 1999;49:67–86 [CrossRef][PubMed]
    [Google Scholar]
  56. Trevisan V. Sul micrococco della rabbia e sulla possiblità di riconoscere durante il periodo d'incubazione, dall'esame del sangue della persona moricata, se ha contratta l'infezione rabbica. Rendiconti dell'Istituto Lombardo di Scienze e Lettere (Ser 2) 1887;20:88–105
    [Google Scholar]
  57. Christensen H, Bisgaard M. The Genus Pasteurella. In Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E et al. (editors) The Prokaryotes, 3rd ed.vol. 6 New York: Springer; 2006; pp.1062–1090[CrossRef]
    [Google Scholar]
  58. Foster G, Ross HM, Malnick H, Willems A, Hutson RA et al. Phocoenobacter uteri gen. nov., sp. nov., a new member of the family Pasteurellaceae pohl (1979) 1981 isolated from a harbour porpoise (Phocoena phocoena). Int J Syst Evol Microbiol 2000;50:135–139 [CrossRef][PubMed]
    [Google Scholar]
  59. Bisgaard M, Korczak BM, Busse HJ, Kuhnert P, Bojesen AM et al. Classification of the taxon 2 and taxon 3 complex of bisgaard within Gallibacterium and description of Gallibacterium melopsittaci sp. nov., Gallibacterium trehalosifermentans sp. nov. and Gallibacterium salpingitidis sp. nov. Int J Syst Evol Microbiol 2009;59:735–744 [CrossRef][PubMed]
    [Google Scholar]
  60. Christensen H, Bisgaard M, Aalbaek B, Olsen JE. Reclassification of Bisgaard taxon 33, with proposal of Volucribacter psittacicida gen. nov., sp. nov. and Volucribacter amazonae sp. nov. as new members of the Pasteurellaceae. Int J Syst Evol Microbiol 2004;54:813–818 [CrossRef][PubMed]
    [Google Scholar]
  61. Blackall PJ, Christensen H, Beckenham T, Blackall LL, Bisgaard M. Reclassification of Pasteurella gallinarum, [Haemophilus] paragallinarum, Pasteurella avium and Pasteurella volantium as Avibacterium gallinarum gen. nov., comb. nov., Avibacterium paragallinarum comb. nov., Avibacterium avium comb. nov. and Avibacterium volantium comb. nov. Int J Syst Evol Microbiol 2005;55:353–362 [CrossRef]
    [Google Scholar]
  62. Blackall PJ, Bojesen AM, Christensen H, Bisgaard M. Reclassification of [Pasteurella] trehalosi as Bibersteinia trehalosi gen. nov., comb. nov. Int J Syst Evol Microbiol 2007;57:666–674 [CrossRef][PubMed]
    [Google Scholar]
  63. Nørskov-Lauritsen N, Kilian M. Reclassification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates. Int J Syst Evol Microbiol 2006;56:2135–2146 [CrossRef][PubMed]
    [Google Scholar]
  64. Kuhnert P, Scholten E, Haefner S, Mayor D, Frey J. Basfia succiniciproducens gen. nov., sp. nov., a new member of the family Pasteurellaceae isolated from bovine rumen. Int J Syst Evol Microbiol 2010;60:44–50 [CrossRef][PubMed]
    [Google Scholar]
  65. Gregersen RH, Neubauer C, Christensen H, Bojesen AM, Hess M et al. Comparative studies on [Pasteurella] testudinis and [P.] testudinis-like bacteria and proposal of Chelonobacter oris gen. nov., sp. nov. as a new member of the family Pasteurellaceae. Int J Syst Evol Microbiol 2009;59:1583–1588 [CrossRef][PubMed]
    [Google Scholar]
  66. Foster G, Higgins R, Leclair D, Korczak BM, Mikaelian I et al. Proposal of Bisgaardia hudsonensis gen. nov., sp. nov. and an additional genomospecies, isolated from seals, as new members of the family Pasteurellaceae. Int J Syst Evol Microbiol 2011;61:3016–3022 [CrossRef][PubMed]
    [Google Scholar]
  67. Hansen MJ, Bertelsen MF, Christensen H, Bojesen AM, Bisgaard M. Otariodibacter oris gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of pinnipeds. Int J Syst Evol Microbiol 2012;62:2572–2578 [CrossRef][PubMed]
    [Google Scholar]
  68. Bisgaard M, Mutters R. Characterization of some previously unclassified "Pasteurella" spp. obtained from the oral cavity of dogs and cats and description of a new species tentatively classified with the family Pasteurellaceae pohl 1981 and provisionally called taxon 16. Acta Pathol Microbiol Immunol Scand B 1986;94:177–184 [CrossRef][PubMed]
    [Google Scholar]
  69. Korczak BM, Bisgaard M, Christensen H, Kuhnert P. Frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds. Antonie van Leeuwenhoek 2014;105:731–741 [CrossRef][PubMed]
    [Google Scholar]
  70. Mühldorfer K, Speck S, Wibbelt G. Proposal of Vespertiliibacter pulmonis gen. nov., sp. nov. and two genomospecies as new members of the family Pasteurellaceae isolated from European bats. Int J Syst Evol Microbiol 2014;64:2424–2430 [CrossRef][PubMed]
    [Google Scholar]
  71. Hansen MJ, Bertelsen MF, Christensen H, Bojesen AM. Bisgaardia miroungae sp. nov., a new member of the family Pasteurellaceae isolated from the oral cavity of northern elephant seals (Mirounga angustirostris), and emended description of the genus Bisgaardia. Int J Syst Evol Microbiol 2015;65:388–392 [CrossRef][PubMed]
    [Google Scholar]
  72. Hansen MJ, Pennanen EA, Bojesen AM, Christensen H, Bertelsen MF. Testudinibacter aquarius gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of freshwater turtles. Int J Syst Evol Microbiol 2016;66:567–573 [CrossRef][PubMed]
    [Google Scholar]
  73. Bisgaard M. Isolation and characterization of some previously unreported taxa from poultry with phenotypical characters related to Actinobacillus-an Pasteurella species. Acta Pathol Microbiol Immunol Scand B 1982;90:59–67 [CrossRef][PubMed]
    [Google Scholar]
  74. Kilian M. A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol 1976;93:9–62 [CrossRef][PubMed]
    [Google Scholar]
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