1887

Abstract

gen. nov. is proposed based on isolation and phenotypic characterization of strains, predominantly from rodents. The strains showed 86 % or higher gene sequence similarity and indicated a genus-level relationship within . The strains compared at 16S rRNA gene sequence level showed 93.8 % or higher similarity, and their genus-level relationship within was confirmed by phenotypic analysis. The type species comb. nov. is reclassified from [] with type strain NCTC 8141 (=CCUG 12398). Whole genomic comparison allowed the estimation of DNA–DNA renaturation. sp. nov. was proposed for a group that included the biovar Heyl of [] with the type strain ATCC 12555 (=CCUG 998). A group was proposed as sp. nov., which included the taxon 22 of Bisgaard; the type strain is F75 (=CCUG 69665=DSM 103977). Taxon 41 of Bisgaard was proposed as sp. nov. with type strain Ac151 (=CCUG 69666=DSM 103994). sp. nov. included the type strain 1996025094 (=Ac69) (=CCUG 69667=DSM 103978). A group strains of was proposed as sp. nov. with type strain H1987082031 (=CCUG 69668=DSM 104075). Two strains including the reference strain of taxon 17 of Bisgaard that showed 16S rRNA gene similarity of 97.3 % were proposed as sp. nov. 2325/79 (=CCUG 17206=DSM 103980). sp. nov. was proposed with type strain Ppn418 (Bisgaard taxon 21) (=CCUG 69669=DSM 103979). The eight species could be separated based on phenotypic characteristics such as NAD requirement, ornithine decarboxylase and indole formation, α-glucosidase, β-galactosidase and in acid formation from (+)--arabinose, (−)--ribose, (+)--xylose, -inositol, (−)--mannitol, lactose, melibiose and trehalose. Forty-six strains including taxon 48 of Bisgaard formed a monophyletic group by and 16S rRNA gene sequence analysis, but could not be separated phenotypically from and , and it was left as an unnamed genomospecies 1 of with reference strain Ppn416. Another taxon that included 13 strains, mainly isolated from , could not be separated phenotypically from or and was designated as genomospecies 2. Strain Ppn85 with 95 % or less gene sequence similarity and with 16S rRNA gene sequence similarity of 97 % or less to the other members of was left as an unnamed singleton.

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