1887

Abstract

Four strains isolated in Iran from pulmonary specimens of unrelated patients are proposed as representative of a novel species. Similarity, at the phenotypic level, with is remarkable with the photochromogenic yellow pigmentation of the colonies being the salient feature. They differ, however, genotypically from this species and present unique sequences in 16S rRNA, and genes. The average nucleotide identity and the genome-to-genome distance fully support the status of an independent species. The name proposed for this species is sp. nov. with AFPC-000227 (=DSM 104278=CIP 111197) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001862
2017-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/6/1766.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001862&mimeType=html&fmt=ahah

References

  1. Buhler VB, Pollak A. Human infection with atypical acid-fast organisms; report of two cases with pathologic findings. Am J Clin Pathol 1953; 23:363–374 [View Article][PubMed]
    [Google Scholar]
  2. Kent PT, Kubica GP. Public Health Mycobacteriology. A Guide for the Level III Laboratory Atlanta: U.S. Department of Health and Human Services; 1985
    [Google Scholar]
  3. Tortoli E, Pecorari M, Fabio G, Messinò M, Fabio A. Commercial DNA probes for mycobacteria incorrectly identify a number of less frequently encountered species. J Clin Microbiol 2010; 48:307–310 [View Article][PubMed]
    [Google Scholar]
  4. CLSI Susceptibility Testing of Mycobacteria, Nocardiae and Other Aerobic Actinomycetes; Approved Standard - Second Edition M24-A2 Wayne, PA: Clinical and Laboratory Standards Institute; 2011
    [Google Scholar]
  5. CDC Standardized Method for HPLC Identification of Mycobacteria Atlanta: U.S. Department of Health and Human Services, Public Health Service; 1996
    [Google Scholar]
  6. Kirschner P, Böttger EC. Species identification of mycobacteria using rDNA sequencing. In Parish T, Stoker NG. (editors) Methods in Molecular Biology Vol. 101 Mycobacteria Protocols Totowa, NJ: Humana Press Inc; 2000 pp. 349–361
    [Google Scholar]
  7. Telenti A, Marchesi F, Balz M, Bally F, Böttger EC et al. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 1993; 31:175–178[PubMed]
    [Google Scholar]
  8. Adékambi T, Colson P, Drancourt M. rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. J Clin Microbiol 2003; 41:5699–5708 [View Article][PubMed]
    [Google Scholar]
  9. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  10. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  11. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
    [Google Scholar]
  12. Lee I, Ouk Kim Y, Chun J, Park S-C. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article]
    [Google Scholar]
  13. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001862
Loading
/content/journal/ijsem/10.1099/ijsem.0.001862
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error