1887

Abstract

The Gram-reaction-negative, aerobic, white- to pale-yellow-coloured and rod-shaped bacterium with a single polar flagellum or a stalk, designated strain 7F14, was isolated from rhizosphere soil of cultivated watermelon (Citrullus lanatus) collected from Hefei, China. Growth of strain 7F14 was observed at pH 6.0–9.0, 10–30 °C and in the presence of 0–1 % (w/v) NaCl. Cells were catalase-negative and oxidase-positive. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain 7F14 formed a phyletic lineage within the genus Caulobacter of the family Caulobacteraceae and showed the highest 16S rRNA gene sequence similarities to Caulobacter henricii ATCC 15253 (98.66 %), Caulobacter segnis ATCC 21756 (98.27 %), Caulobacter vibrioides CB51 (97.92 %) and C aulobacter flavus RHGG3 (97.44 %). The G+C content of the genomic DNA was 68.6 mol%. Strain 7F14 contained Q-10 as the sole ubiquinone and 11-methyl C18 : 1ω7c, C18 : 1ω7c, C16 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) as the major fatty acids. The polar lipids profile consisted of phosphatidylglycerol, an unknown phosphoglycolipid, five unknown glycolipids, an unknown phospholipid and three unknown lipids. DNA–DNA relatedness values to the most closely related type strains Caulobacter henricii DSM 4730 and Caulobacter segnis DSM 7131 were 26.0 and 19.7 %, respectively. Based on unique phenotypic traits, and phylogenetic, chemotaxonomic and DNA–DNA hybridization results, strain 7F14 should be classified as a representative of a novel species of the genus Caulobacter , for which the name C aulobacter rhizosphaerae sp. nov. is proposed. The type strain is 7F14 (=CGMCC 1.15915=KCTC 52515).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001860
2017-06-09
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/6/1771.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001860&mimeType=html&fmt=ahah

References

  1. Henrici AT, Johnson DE. Studies of freshwater bacteria: II. Stalked bacteria, a new order of Schizomycetes. J Bacteriol 1935;30:61–93[PubMed]
    [Google Scholar]
  2. Bowers LE, Weaver RH, Grula EA, Edwards OF. Studies on a strain of Caulobacter from water. I. Isolation and identification as Caulobacter vibrioides Henrici and Johnson with emended description. J Bacteriol 1954;68:194–200[PubMed]
    [Google Scholar]
  3. Poindexter JS. Biological properties and classification of the Caulobacter group. Bacteriol Rev 1964;28:231–295[PubMed]
    [Google Scholar]
  4. Abraham WR, Strömpl C, Meyer H, Lindholst S, Moore ER et al. Phylogeny and polyphasic taxonomy of Caulobacter species. proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) comb. nov. as the type species, and emended description of the genera Brevundimonas and Caulobacter. Int J Syst Bacteriol 1999;49:1053–1073 [CrossRef][PubMed]
    [Google Scholar]
  5. Stahl DA, Key R, Flesher B, Smit J. The phylogeny of marine and freshwater caulobacters reflects their habitat. J Bacteriol 1992;174:2193–2198 [CrossRef][PubMed]
    [Google Scholar]
  6. Chen H, Jogler M, Rohde M, Klenk HP, Busse HJ et al. Reclassification and emended description of Caulobacter leidyi as Sphingomonas leidyi comb. nov., and emendation of the genus Sphingomonas. Int J Syst Evol Microbiol 2012;62:2835–2843 [CrossRef][PubMed]
    [Google Scholar]
  7. Urakami T, Oyanagi H, Araki H, Suzuki K-I, Komagata K. Recharacterization and emended description of the genus Mycoplana and description of two new species, Mycoplana ramosa and Mycoplana segnis. Int J Syst Bacteriol 1990;40:434–442 [CrossRef]
    [Google Scholar]
  8. Poindexter JS, Lewis RF. Recommendations for revision of the taxonomic treatment of stalked bacteria. Int J Syst Bacteriol 1966;16:377–382 [CrossRef]
    [Google Scholar]
  9. Abraham WR, Macedo AJ, Lünsdorf H, Fischer R, Pawelczyk S et al. Phylogeny by a polyphasic approach of the order Caulobacterales, proposal of Caulobacter mirabilis sp. nov., Phenylobacterium haematophilum sp. nov. and Phenylobacterium conjunctum sp. nov., and emendation of the genus Phenylobacterium. Int J Syst Evol Microbiol 2008;58:1939–1949 [CrossRef][PubMed]
    [Google Scholar]
  10. Liu QM, Ten LN, Im WT, Lee ST, Yoon MH. Caulobacter ginsengisoli sp. nov., a novel stalked bacterium isolated from ginseng cultivating soil. J Microbiol Biotechnol 2010;20:15–20[PubMed]
    [Google Scholar]
  11. Jin L, Lee HG, Kim HS, Ahn CY, Oh HM. Caulobacter daechungensis sp. nov., a stalked bacterium isolated from a eutrophic reservoir. Int J Syst Evol Microbiol 2013;63:2559–2564 [CrossRef][PubMed]
    [Google Scholar]
  12. Jin L, La HJ, Lee HG, Lee JJ, Lee S et al. Caulobacter profunda sp. nov., isolated from deep freshwater sediment. Int J Syst Evol Microbiol 2014;64:762–767 [CrossRef][PubMed]
    [Google Scholar]
  13. Sun LN, Yang ED, Wei JC, Tang XY, Cao YY et al. Caulobacter flavus sp. nov., a stalked bacterium isolated from rhizosphere soil. Int J Syst Evol Microbiol 2015;65:4374–4380 [CrossRef][PubMed]
    [Google Scholar]
  14. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM. et al. (editors) Methods for General and Molecular Microbiology Washington, DC: American Society for Microbiology; 2007; pp.330–393
    [Google Scholar]
  15. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987;19:1–67
    [Google Scholar]
  16. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  17. Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA et al. Clustal W and clustal X version 2.0. Bioinformatics 2007;23:2947–2948 [CrossRef][PubMed]
    [Google Scholar]
  18. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  19. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  20. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  21. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  22. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  23. de Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970;12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  24. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006;33:152–155
    [Google Scholar]
  25. Meier-Kolthoff JP, Göker M, Spröer C, Klenk HP. When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 2013;195:413–418 [CrossRef][PubMed]
    [Google Scholar]
  26. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014;64:346–351 [CrossRef][PubMed]
    [Google Scholar]
  27. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987;37:463–464[CrossRef]
    [Google Scholar]
  28. Miller LT. A single derivatization method for bacterial fatty acid methyl esters including hydroxy acids. J Clin Microbiol 1982;16:584–586
    [Google Scholar]
  29. Kuykendall LD, Roy MA, O'Neill JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 1988;38:358–361 [CrossRef]
    [Google Scholar]
  30. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990;13:128–130 [CrossRef]
    [Google Scholar]
  31. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990;66:199–202[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001860
Loading
/content/journal/ijsem/10.1099/ijsem.0.001860
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error