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Abstract

A Gram-staining-negative, non-spore-forming, non-motile, rod-shaped, facultatively anaerobe bacterial strain, designated CAU 1312, was isolated from sea sand of Eurwangri beach, South Korea. The strain’s taxonomic position was investigated using a polyphasic approach. CAU 1312 grew at temperatures from 20 to 40 °C, in the range of pH 6.0–9.0 and at salinities from 1–4 % (w/v). The results of phylogenetic analysis based on the 16S rRNA gene sequence revealed that CAU 1312 represented a member of the genus Thioclava and was most closely related to Thioclava atlantica 13D2W-2 (similarity 96.53 %). The strain contained Q-10 as the predominant menaquinone and summed feature 8 (C18 : 1ω7c/ω6c) as the major fatty acid. The polar lipids of CAU 1312 consisted of phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, a phosphoglycolipid, and two unidentified phospholipids. The DNA G+C content was 64.7 mol%. On the basis of phenotypic and chemotaxonomic properties and phylogenetic inference, CAU 1312 is considered to represent a novel species of the genus Thioclava , for which the name Thioclava arenosa sp. nov. is proposed. The type strain is CAU 1312(=KCTC 52190=NBRC 111989).

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2017-06-09
2019-10-15
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