@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001850, author = "Lee, Jerry Y. and Iglesias, Brenda and Chu, Caleb E. and Lawrence, Daniel J. P. and Crane III, Edward Jerome", title = "Pyrobaculum igneiluti sp. nov., a novel anaerobic hyperthermophilic archaeon that reduces thiosulfate and ferric iron", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "6", pages = "1714-1719", doi = "https://doi.org/10.1099/ijsem.0.001850", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001850", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "mud volcano", keywords = "Pyrobaculum", keywords = "hyperthermophile", keywords = "Fe(III) respiration", keywords = "thiosulfate", abstract = "A novel anaerobic, hyperthermophilic archaeon was isolated from a mud volcano in the Salton Sea geothermal system in southern California, USA. The isolate, named strain 521T, grew optimally at 90 °C, at pH 5.5–7.3 and with 0–2.0 % (w/v) NaCl, with a generation time of 10 h under optimal conditions. Cells were rod-shaped and non-motile, ranging from 2 to 7 µm in length. Strain 521T grew only in the presence of thiosulfate and/or Fe(III) (ferrihydrite) as terminal electron acceptors under strictly anaerobic conditions, and preferred protein-rich compounds as energy sources, although the isolate was capable of chemolithoautotrophic growth. 16S rRNA gene sequence analysis places this isolate within the crenarchaeal genus Pyrobaculum . To our knowledge, this is the first Pyrobaculum strain to be isolated from an anaerobic mud volcano and to reduce only either thiosulfate or ferric iron. An in silico genome-to-genome distance calculator reported <25 % DNA–DNA hybridization between strain 521T and eight other Pyrobaculum species. Due to its genotypic and phenotypic differences, we conclude that strain 521T represents a novel species, for which the name Pyrobaculum igneiluti sp. nov. is proposed. The type strain is 521T (=DSM 103086T=ATCC TSD-56T).", }