@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001849, author = "Zhang, Gaiyun and Wang, Lina and Li, Jinglong and Zhou, Yuan", title = "Pseudonocardia profundimaris sp. nov., isolated from marine sediment", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "6", pages = "1693-1697", doi = "https://doi.org/10.1099/ijsem.0.001849", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001849", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Pseudonocardia profundimaris sp. nov.", keywords = "polyphasic approach", abstract = "A novel Gram-stain-positive, aerobic, non-motile actinobacterium, designated GY0556T, was isolated from deep-sea sediment collected from the western Pacific Ocean at a depth of 7118 m. Phylogenetic analysis based on 16S rRNA gene sequences revealed that this strain belongs to the genus Pseudonocardia , being most closely related to Pseudonocardia hydrocarbonoxydans IMSNU 22140T (97.6 % similarity), Pseudonocardia sulfidoxydans DSM 44248T (97.6 %) and Pseudonocardia alaniniphila Y-16303T (97.6 %); similarity to other type strains of the genus Pseudonocardia was less than 97.5 %. Strain GY0556T contained MK-8(H4) as the predominant menaquinone and iso-C16 : 0 and iso H-C16 : 1 as the major fatty acids. The polar lipids detected in strain GY0556T were phosphatidylcholine, phosphatidylinositol, one unknown glycolipid, one unknown phospholipid and two unknown lipids. The whole organism hydrolysates mainly consisted of meso-diaminopimelic acid, xylose, galactose and arabinose. The DNA G+C content of strain GY0556T was 76.9 mol%. The results of DNA–DNA hybridizations and phylogenetic analysis, together with the phenotypic and biochemical tests, allowed the differentiation of strain GY0556T from established members of the genus Pseudonocardia . Therefore, it is proposed that strain GY0556T represents a novel species of the genus Pseudonocardia , for which the name Pseudonocardia profundimaris sp. nov. is proposed. The type strain is GY0556T (=MCCC 1A10574T=KCTC 39641T).", }