1887

Abstract

A Gram-stain-negative, non-motile strain, WCHA30, was isolated from hospital sewage in West China Hospital of Sichuan University in Chengdu, south-western China. Strain WCHA30 was a non-spore-forming, catalase-positive, oxidase-negative, strictly aerobic coccobacillus. The DNA G+C content was 38 mol%. Phylogenetic analysis based on 16S rRNA, and gene sequences revealed that the strain was distinct from any previously described species of the genus . Strain WCHA30 could be distinguished from all known species by its ability to assimilate β-alanine but not -glutamate. Genotypic and phenotypic characteristics from this study indicate that strain WCHA30 should be considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WCHA30 (=CCTCC AB 2016203=GDMCC 1.1101=KCTC 52503).

Keyword(s): Acinetobacter , genome and sewage
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001847
2017-06-01
2020-01-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/6/1709.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001847&mimeType=html&fmt=ahah

References

  1. Brisou J, Prevot AR. Studies on bacterial taxonomy. X. The revision of species under Acromobacter group. Ann Inst Pasteur 1954;86:722–728[PubMed]
    [Google Scholar]
  2. Bernards AT, de Beaufort AJ, Dijkshoorn L, van Boven CP. Outbreak of septicaemia in neonates caused by Acinetobacter junii investigated by amplified ribosomal DNA restriction analysis (ARDRA) and four typing methods. J Hosp Infect 1997;35:129–140 [CrossRef][PubMed]
    [Google Scholar]
  3. Nemec A, Dijkshoorn L, Cleenwerck I, de Baere T, Janssens D et al. Acinetobacter parvus sp. nov., a small-colony-forming species isolated from human clinical specimens. Int J Syst Evol Microbiol 2003;53:1563–1567 [CrossRef][PubMed]
    [Google Scholar]
  4. Nemec A, Dijkshoorn L, Jezek P. Recognition of two novel phenons of the genus Acinetobacter among non-glucose-acidifying isolates from human specimens. J Clin Microbiol 2000;38:3937–3941[PubMed]
    [Google Scholar]
  5. Nemec A, Musílek M, Maixnerová M, de Baere T, van der Reijden TJ et al. Acinetobacter beijerinckii sp. nov. and Acinetobacter gyllenbergii sp. nov., haemolytic organisms isolated from humans. Int J Syst Evol Microbiol 2009;59:118–124 [CrossRef][PubMed]
    [Google Scholar]
  6. Anandham R, Weon HY, Kim SJ, Kim YS, Kim BY et al. Acinetobacter brisouii sp. nov., isolated from a wetland in Korea. J Microbiol 2010;48:36–39 [CrossRef][PubMed]
    [Google Scholar]
  7. Carr EL, Kämpfer P, Patel BK, Gürtler V, Seviour RJ. Seven novel species of Acinetobacter isolated from activated sludge. Int J Syst Evol Microbiol 2003;53:953–963 [CrossRef][PubMed]
    [Google Scholar]
  8. Choi JY, Ko G, Jheong W, Huys G, Seifert H et al. Acinetobacter kookii sp. nov., isolated from soil. Int J Syst Evol Microbiol 2013;63:4402–4406 [CrossRef][PubMed]
    [Google Scholar]
  9. Krizova L, Maixnerova M, Sedo O, Nemec A. Acinetobacter bohemicus sp. nov. widespread in natural soil and water ecosystems in the Czech Republic. Syst Appl Microbiol 2014;37:467–473 [CrossRef][PubMed]
    [Google Scholar]
  10. Li W, Zhang D, Huang X, Qin W. Acinetobacter harbinensis sp. nov., isolated from river water. Int J Syst Evol Microbiol 2014;64:1507–1513 [CrossRef][PubMed]
    [Google Scholar]
  11. Vaneechoutte M, Nemec A, Musílek M, van der Reijden TJ, van den Barselaar M et al. Description of Acinetobacter venetianus ex Di Cello et al. 1997 sp. nov. Int J Syst Evol Microbiol 2009;59:1376–1381 [CrossRef][PubMed]
    [Google Scholar]
  12. Vaz-Moreira I, Novo A, Hantsis-Zacharov E, Lopes AR, Gomila M et al. Acinetobacter rudis sp. nov., isolated from raw milk and raw wastewater. Int J Syst Evol Microbiol 2011;61:2837–2843 [CrossRef][PubMed]
    [Google Scholar]
  13. CLSI Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement. M100-S23 Wayne, PA, USA: Clinical and Laboratory Standards Institute; 2013
    [Google Scholar]
  14. Zong Z, Zhang X. blaNDM-1-carrying Acinetobacter johnsonii detected in hospital sewage. J Antimicrob Chemother 2013;68:1007–1010 [CrossRef][PubMed]
    [Google Scholar]
  15. Lane D. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester, UK: John Wiley and Sons; 1991; pp.115–175
    [Google Scholar]
  16. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  17. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  18. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012;19:455–477 [CrossRef][PubMed]
    [Google Scholar]
  19. Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 2014;30:2068–2069 [CrossRef][PubMed]
    [Google Scholar]
  20. Larsen MV, Cosentino S, Rasmussen S, Friis C, Hasman H et al. Multilocus sequence typing of total-genome-sequenced Bacteria. J Clin Microbiol 2012;50:1355–1361 [CrossRef][PubMed]
    [Google Scholar]
  21. Sievers F, Wilm A, Dineen D, Gibson TJ, Karplus K et al. Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol 2011;7:539 [CrossRef][PubMed]
    [Google Scholar]
  22. Chan JZ, Halachev MR, Loman NJ, Constantinidou C, Pallen MJ. Defining bacterial species in the genomic era: insights from the genus Acinetobacter. BMC Microbiol 2012;12:302 [CrossRef][PubMed]
    [Google Scholar]
  23. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009;106:19126–19131 [CrossRef][PubMed]
    [Google Scholar]
  24. De Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970;12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  25. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14:60 [CrossRef][PubMed]
    [Google Scholar]
  26. Krizova L, Maixnerova M, Sedo O, Nemec A. Acinetobacter albensis sp. nov., isolated from natural soil and water ecosystems. Int J Syst Evol Microbiol 2015;65:3905–3912 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001847
Loading
/content/journal/ijsem/10.1099/ijsem.0.001847
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error